Sabbatini G P, von Holt C
UCT-CSIR Research Centre for Molecular Biology, Department of Biochemistry, University of Cape Town, Republic of South Africa.
FEBS Lett. 1987 Nov 16;224(1):117-20. doi: 10.1016/0014-5793(87)80432-5.
We found 8-azidoadenosine 5'-diphosphate to be a phosphoryl acceptor in the enzymatic conversion of 1,3-diphosphoglyceric acid to 3-phosphoglycerate. This has allowed us to synthesize in a single-step procedure carrier-free 8-azidoadenosine 5'-[gamma-32P]triphosphate, requiring no further purification of the end product. The synthesized 8-azidoadenosine 5'-[gamma-32P]triphosphate has been characterized and shown to meet all the criteria for a specific photoreactive ATP analogue.
我们发现8-叠氮腺苷5'-二磷酸是1,3-二磷酸甘油酸酶促转化为3-磷酸甘油酸过程中的磷酰受体。这使我们能够通过一步法合成无载体的8-叠氮腺苷5'-[γ-32P]三磷酸，无需对终产物进行进一步纯化。所合成的8-叠氮腺苷5'-[γ-32P]三磷酸已得到表征，并显示符合特定光反应性ATP类似物的所有标准。
