Fendler K, Grell E, Bamberg E
Max-Planck-Institut für Biophysik, Frankfurt am Main, FRG.
FEBS Lett. 1987 Nov 16;224(1):83-8. doi: 10.1016/0014-5793(87)80427-1.
Purified Na+,K+-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s-1 (22 degrees C). The exponential components found in the transient currents are compared to transitions in the Albers-Post scheme.
从猪肾中纯化得到的钠钾ATP酶被附着于黑色脂质膜上。通过用紫外激光脉冲释放笼形ATP来释放ATP，该酶的泵电流能够以大约1毫秒的时间分辨率进行测量。对瞬态电流的分析表明，一个缓慢的非电生步骤之后是一个电生转变，其速率常数为100 s⁻¹（22摄氏度）。将瞬态电流中发现的指数成分与阿尔伯斯-波斯特模型中的转变进行了比较。
