Shafaroodi Hamed, Shahbek Farnaz, Faizi Mehrdad, Ebrahimi Farzad, Moezi Leila
Department of Pharmacology and Toxicology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. ; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Toxicology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Iran J Pharm Res. 2016 Fall;15(4):843-850.
Creatine exerts beneficial effects on a variety of pathologies in which energy metabolism and oxidative stress play an etiological role. Creatine supplements have shown beneficial effects on neurological disorders including Parkinson׳s disease, Huntington›s disease, amyotrophic lateral sclerosis, as well as Alzheimer›s disease and stroke. However, the potential benefits of creatine for patients with convulsive disorders remain poorly defined. While some authors did not suggest any anti- or pro-convulsant roles for creatine treatment, others suggest that creatine may be an anticonvulsant agent. In this study, we investigated the effects of creatine on seizures in mice. Three models were used to explore the role of creatine on seizures in mice including intravenous pentylenetetrazole (PTZ), intraperitoneal PTZ, and electroshock models. Acute creatine treatment (10, 20, 40 and 80 mg/Kg) significantly increased the clonic seizure threshold in the intravenous PTZ model. Sub-chronic administration of creatine (10 and 20 mg/Kg) revealed a significant anticonvulsant effect in intravenous PTZ model. Acute creatine administration (10, 20 and 40 mg/Kg) significantly decreased the frequency of clonic seizures in the intraperitoneal PTZ model. Besides, acute creatine (40 and 80 mg/Kg) decreased the incidence of tonic seizures after electroshock. In conclusion, creatine exerts anticonvulsant effects in three seizure models; therefore, it may act as a potential drug to help patients with convulsions. However, further investigations should be done to clarify these results more.
肌酸对多种能量代谢和氧化应激起病因学作用的病理状况具有有益影响。肌酸补充剂已显示出对包括帕金森病、亨廷顿病、肌萎缩侧索硬化症以及阿尔茨海默病和中风在内的神经系统疾病具有有益作用。然而，肌酸对惊厥性疾病患者的潜在益处仍未明确界定。虽然一些作者未表明肌酸治疗有任何抗惊厥或促惊厥作用，但其他作者认为肌酸可能是一种抗惊厥剂。在本研究中，我们研究了肌酸对小鼠癫痫发作的影响。使用了三种模型来探究肌酸在小鼠癫痫发作中的作用，包括静脉注射戊四氮（PTZ）、腹腔注射PTZ和电休克模型。急性肌酸治疗（10、20、40和80毫克/千克）显著提高了静脉注射PTZ模型中的阵挛性癫痫发作阈值。亚慢性给予肌酸（10和20毫克/千克）在静脉注射PTZ模型中显示出显著的抗惊厥作用。急性给予肌酸（10、20和40毫克/千克）显著降低了腹腔注射PTZ模型中阵挛性癫痫发作的频率。此外，急性给予肌酸（40和80毫克/千克）降低了电休克后强直性癫痫发作的发生率。总之，肌酸在三种癫痫发作模型中发挥抗惊厥作用；因此，它可能作为一种潜在药物来帮助惊厥患者。然而，应进行进一步研究以更清楚地阐明这些结果。
