Liu Xiaochen, Walawage Sriema L, Leslie Charles A, Dandekar Abhaya M, Tricoli David M, Hu Hengkang, Huang Youjun, Zhang Jiaqi, Xv Chuanmei, Huang Jianqin, Zhang Qixiang
School of Forestry and Biotechnology, Zhejiang A&F University, Lin'an, 311300, Zhejiang, China.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, 311300, Zhejiang, China.
Plant Cell Rep. 2017 Jun;36(6):877-885. doi: 10.1007/s00299-017-2116-1. Epub 2017 Feb 27.
An in vitro grafting method was developed for examining gene translocation from rootstock to scion in walnut. Results showed the DsRED gene itself was not translocated but expressed mRNA was. Grafting is widely used in plants, especially in fruit and nut crops. Selected rootstocks can control scion growth and physiological traits, including shortening of the juvenile phase and controlling tree size. Rootstocks also can provide improved soil adaptation and pathogen resistance. Development of genetically modified (GM) fruit crops has progressed recently, but commercial cultivation is still limited due to the time required for evaluation and issues with deregulation. In this study, we evaluated the stability of DsRED marker gene expression in in vitro walnut shoots and examined translocation of the gene and its mRNA from transformed rootstock to wild-type scion. Results show that DsRED was expressed uniformly in transformed tissue-cultured shoots. When used as in vitro rootstocks, these had good graft affinity with wild-type control scion. PCR and qRT-PCR analysis showed that the DsRED gene was not transported from rootstock to scion, but the transcribed mRNA was translocated. This result provides further evidence of gene signal transport from rootstock to scion in fruit and nut crops.
我们开发了一种体外嫁接方法，用于检测核桃中基因从砧木向接穗的转移。结果表明，DsRED基因本身并未转移，但转录的mRNA发生了转移。嫁接在植物中广泛应用，尤其是在果树和坚果树作物中。选定的砧木可以控制接穗的生长和生理特性，包括缩短幼年期和控制树体大小。砧木还能增强土壤适应性和抗病性。近年来，转基因水果作物的研发取得了进展，但由于评估所需时间和监管问题，商业种植仍然有限。在本研究中，我们评估了体外培养的核桃嫩枝中DsRED标记基因表达的稳定性，并检测了该基因及其mRNA从转基因砧木向野生型接穗的转移情况。结果显示，DsRED在转化的组培嫩枝中表达一致。当用作体外砧木时，它们与野生型对照接穗具有良好的嫁接亲和力。PCR和qRT-PCR分析表明，DsRED基因未从砧木转移至接穗，但转录的mRNA发生了转移。这一结果为果树和坚果树作物中基因信号从砧木向接穗的运输提供了进一步证据。
