Centola M B, Tsai M M, Deonier R C
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1481.
J Bacteriol. 1987 Dec;169(12):5852-5. doi: 10.1128/jb.169.12.5852-5855.1987.
Tn1000 (gamma delta) termini IRR and IRL, or direct repetitions of IRR-IRL carried by pBR322 derivatives mediate cointegration with pOX38 at similar rates. Structures of product plasmids indicate that the transposed segments correspond to DNA bounded by IR segments in the donor plasmid. Such structures could arise by symmetric transposition from a replication intermediate.
Tn1000(γδ)末端的IRR和IRL,或pBR322衍生物携带的IRR-IRL直接重复序列,以相似的速率介导与pOX38的共整合。产物质粒的结构表明,转座片段对应于供体质粒中由IR片段界定的DNA。这种结构可能由复制中间体的对称转座产生。