Nathan Sarah R, Pino Nicholas W, Arduino Daniela M, Perocchi Fabiana, MacMillan Samantha N, Wilson Justin J
Department of Chemistry and Chemical Biology, Cornell University , Ithaca, New York 14853, United States.
Gene Center, Department of Biochemistry, Ludwig-Maximilans Universität München , Munich, Germany.
Inorg Chem. 2017 Mar 20;56(6):3123-3126. doi: 10.1021/acs.inorgchem.6b03108. Epub 2017 Feb 28.
The mixed-valent oxo-bridged ruthenium complex [(HCO)(NH)Ru(μ-O)Ru(NH)(OCH)], known as Ru360, is a selective inhibitor of mitochondrial calcium uptake. Although this compound is useful for studying the role of mitochondrial calcium in biological processes, its widespread availability is limited because of challenges in purification and characterization. Here, we describe our investigations of three different synthetic methods for the preparation of a functional analogue of this valuable compound. We demonstrate that this analogue, isolated from our procedures, exhibits potent mitochondrial calcium uptake inhibitory properties in permeabilized HeLa cells and in isolated mitochondria.
混合价态的氧桥联钌配合物[(HCO)(NH)Ru(μ-O)Ru(NH)(OCH)]，即Ru360，是线粒体钙摄取的选择性抑制剂。尽管该化合物对于研究线粒体钙在生物过程中的作用很有用，但由于纯化和表征方面的挑战，其广泛可得性受到限制。在此，我们描述了对三种不同合成方法的研究，以制备这种有价值化合物的功能类似物。我们证明，从我们的实验步骤中分离出的这种类似物，在通透的HeLa细胞和分离的线粒体中表现出强大的线粒体钙摄取抑制特性。
