Mangolim Camila Sampaio, Silva Thamara Thaiane da, Fenelon Vanderson Carvalho, Koga Luciana Numata, Ferreira Sabrina Barbosa de Souza, Bruschi Marcos Luciano, Matioli Graciette
Postgraduate Program in Food Science, State University of Maringá (UEM), Maringá, Paraná, Brazil.
Department of Food Engineering, State University of Maringá (UEM), Maringá, Paraná, Brazil.
PLoS One. 2017 Feb 28;12(2):e0171469. doi: 10.1371/journal.pone.0171469. eCollection 2017.
Curdlan is a linear polysaccharide considered a dietary fiber and with gelation properties. This study evaluated the structure, morphology and the physicochemical and technological properties of curdlan produced by Agrobacterium sp. IFO 13140 recovered by pre-gelation and precipitation methods. Commercial curdlan submitted or otherwise to the pre-gelation process was also evaluated. The data obtained from structural analysis revealed a similarity between the curdlan produced by Agrobacterium sp. IFO 13140 (recovered by both methods) and the commercial curdlans. The results showed that the curdlans evaluated differed significantly in terms of dispersibility and gelation, and only the pre-gelled ones had significant potential for food application, because this method influence on the size of the particles and in the presence of NaCl. In terms of technological properties, the curdlan produced by Agrobacterium sp. IFO 13140 (pre-gelation method) had a greater water and oil holding capacity (64% and 98% greater, respectively) and a greater thickening capacity than the pre-gelled commercial curdlan. The pre-gelled commercial curdlan displayed a greater gelling capacity at 95°C than the others. When applied to food, only the pre-gelled curdlans improved the texture parameters of yogurts and reduced syneresis. The curdlan gels, which are rigid and stable in structure, demonstrated potential for improving the texture of food products, with potential industrial use.
凝胶多糖是一种被视为膳食纤维且具有凝胶特性的线性多糖。本研究评估了通过预凝胶化和沉淀法回收的土壤杆菌IFO 13140产生的凝胶多糖的结构、形态以及物理化学和工艺特性。还对经过或未经过预凝胶化处理的市售凝胶多糖进行了评估。从结构分析获得的数据表明,土壤杆菌IFO 13140产生的凝胶多糖(通过两种方法回收)与市售凝胶多糖之间存在相似性。结果表明,所评估的凝胶多糖在分散性和凝胶化方面存在显著差异,只有经过预凝胶化处理的凝胶多糖具有显著的食品应用潜力,因为这种方法会影响颗粒大小和氯化钠的存在。在工艺特性方面,土壤杆菌IFO 13140产生的凝胶多糖(预凝胶化法)比经过预凝胶化处理的市售凝胶多糖具有更大的持水和持油能力(分别高出64%和98%)以及更大的增稠能力。经过预凝胶化处理的市售凝胶多糖在95°C时显示出比其他凝胶多糖更大的凝胶化能力。当应用于食品时,只有经过预凝胶化处理的凝胶多糖改善了酸奶的质地参数并减少了脱水收缩。结构刚性且稳定的凝胶多糖凝胶显示出改善食品质地的潜力,具有潜在的工业用途。