Futamura K, Maruo T, Mochizuki M
Department of Obstetrics and Gynecology, Kobe University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Sep;39(9):1641-8.
The effects of dibutyryl cyclic adenosine 3',5'-monophosphate (cAMP) and epidermal growth factor (EGF) on the synthesis and secretion of human chorionic gonadotropin (hCG) and its subunits by normal and malignant trophoblasts as well as by non-trophoblastic cells were investigated in vitro. The explants of normal early placental tissues, choriocarcinoma cell line BeWo and non-trophoblastic tumor cell line CaSki from epidermoid carcinoma of the cervix, respectively, were cultured in the presence or absence of dibutyryl cAMP or EGF. The addition of either dibutyryl cAMP (1 mM) or EGF (100 ng/ml) caused significant increases in the synthesis and secretion of hCG and its subunits in cultures of normal and malignant trophoblasts, but had no stimulatory effect on hCG beta synthesis and secretion in culture of non-trophoblastic cell line CaSki that secretes predominantly hCG beta-like material. The magnitude of the stimulatory effects of dibutyryl cAMP and EGF on hCG (alpha,beta) synthesis and secretion by BeWo cells was much greater than that observed in normal trophoblasts. The time course of these stimulatory effects indicated that EGF-stimulated increase in hCG synthesis and secretion required a lag period longer than that for the dibutyryl cAMP-stimulated increase. These results suggest that there were no differences in normal and malignant trophoblasts in the mechanism for the stimulatory regulation of hCG (alpha, beta) synthesis and secretion, but immunoreactive hCG beta synthesis and secretion in non-trophoblastic tumor cells are regulated by a mechanism different from that in trophoblastic cells.
在体外研究了二丁酰环磷腺苷（cAMP）和表皮生长因子（EGF）对正常和恶性滋养层细胞以及非滋养层细胞合成和分泌人绒毛膜促性腺激素（hCG）及其亚基的影响。分别将正常早期胎盘组织、绒毛膜癌细胞系BeWo和宫颈表皮样癌的非滋养层肿瘤细胞系CaSki的外植体在有或无二丁酰cAMP或EGF的情况下进行培养。添加二丁酰cAMP（1 mM）或EGF（100 ng/ml）可使正常和恶性滋养层细胞培养物中hCG及其亚基的合成和分泌显著增加，但对主要分泌hCGβ样物质的非滋养层细胞系CaSki培养物中的hCGβ合成和分泌没有刺激作用。二丁酰cAMP和EGF对BeWo细胞hCG（α，β）合成和分泌的刺激作用程度远大于在正常滋养层细胞中观察到的程度。这些刺激作用的时间进程表明，EGF刺激的hCG合成和分泌增加所需的延迟期比二丁酰cAMP刺激的增加所需的延迟期更长。这些结果表明，正常和恶性滋养层细胞在hCG（α，β）合成和分泌的刺激调节机制上没有差异，但非滋养层肿瘤细胞中免疫反应性hCGβ的合成和分泌受与滋养层细胞不同的机制调节。
