Javed Rabyia, Taku A K, Sharma R K, Badroo Gulzaar Ahmed
Department of Microbiology, Faculty of Veterinary Sciences & Animal Husbandry, R.S. Pura, Sher-E-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India.
Vet World. 2017 Jan;10(1):6-10. doi: 10.14202/vetworld.2017.6-10. Epub 2017 Jan 5.
The aim was to determine the occurrence of in equines and their environment in Jammu (R.S. Pura, Katra), molecular characterization and to determine the antibiotic resistance pattern of .
A total of 96 nasopharyngeal swab samples were collected from equines. The organism was isolated on Columbia nalidixic acid agar containing 5% sheep blood as well as on sheep blood agar and was later confirmed by cultural characteristics and biochemical tests. Molecular detection of isolates was done by gene amplification followed by virulence associated protein A () gene amplification. Antibiogram was performed against five antibiotics, ., amoxicillin, penicillin G, streptomycin, rifampicin, and methicillin.
During the study, 9 isolates were identified on the basis of cultural and biochemical tests. In the polymerase chain reaction based detection, 3 among the 9 rhodococcal isolates were positive for species-specific gene and revealed amplicon of 450 bp for confirmation of gene. None of the sample was found positive for gene. In antibiogram, isolates were found sensitive for amoxicillin, while some isolates were also found resistant to the most conventional antibiotic penicillin G.
From this study, it was concluded that infection is prevalent in equines in Jammu region of India and the indiscriminate use of the antibiotics is leading toward the development of resistant strains of .
确定查谟（R.S.普拉、卡特拉）马及其环境中[具体微生物名称未给出]的存在情况、进行分子特征分析并确定[具体微生物名称未给出]的抗生素耐药模式。
从马身上共采集96份鼻咽拭子样本。该微生物在含5%羊血的哥伦比亚萘啶酸琼脂以及羊血琼脂上分离，随后通过培养特征和生化试验进行确认。对[具体微生物名称未给出]分离株进行分子检测，先进行[具体基因名称未给出]基因扩增，接着进行毒力相关蛋白A（[具体基因名称未给出]）基因扩增。针对五种抗生素，即阿莫西林、青霉素G、链霉素、利福平及甲氧西林进行药敏试验。
在研究过程中，根据培养和生化试验鉴定出9株[具体微生物名称未给出]分离株。在基于聚合酶链反应的检测中，9株红球菌分离株中有3株[具体微生物名称未给出]种特异性[具体基因名称未给出]基因呈阳性，显示出450 bp的扩增子以确认[具体基因名称未给出]基因。未发现样本对[具体基因名称未给出]基因呈阳性。在药敏试验中，[具体微生物名称未给出]分离株对阿莫西林敏感，而一些分离株也对最常用的抗生素青霉素G耐药。
从本研究得出结论，印度查谟地区马中[具体微生物名称未给出]感染普遍存在，抗生素的滥用正导致[具体微生物名称未给出]耐药菌株的产生。
