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蛋白质羽毛硫酸水解的动力学研究

Kinetic study of sulphuric acid hydrolysis of protein feathers.

作者信息

Ben Hamad Bouhamed Sana, Kechaou Nabil

机构信息

Groupe de recherche en Génie des Procédés Agroalimentaires, Laboratoire de recherche en Mécanique des Fluides Appliquée - Génie des Procédés - Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038, Sfax, Tunisia.

出版信息

Bioprocess Biosyst Eng. 2017 May;40(5):715-721. doi: 10.1007/s00449-017-1737-7. Epub 2017 Feb 28.

Abstract

Poultry feather keratin is the most important by-product from the poultry industry due to its abundance. Different methods have been still applied to process this by-product such as enzymatic hydrolysis which is expensive and inapplicable at the industrial level. This paper presents a study of acid hydrolysis of poultry feathers using different types of acids, sulphuric acid concentration, different temperatures and solid to liquid ratio to obtain a liquid product rich in peptides. The feathers analysis revealed a crude protein content of 88.83%. A maximum peptides production of 676 mg/g was reached using sulphuric acid, 1 molar acid concentration and 50 g/l solid to liquid ratio at a temperature of 90 °C after 300 min. A reaction scheme for protein aggregation and decomposition to polypeptides and amino acids was proposed and a kinetic model for peptides production was developed. The proposed kinetic model proved to be well adapted to the experimental data with R  = 0.99.

摘要

家禽羽毛角蛋白因其产量丰富,是家禽行业最重要的副产品。仍有不同方法用于处理该副产品,如酶水解,但酶水解成本高昂且不适用于工业规模。本文研究了使用不同类型的酸、硫酸浓度、不同温度和固液比进行家禽羽毛酸水解,以获得富含肽的液体产物。羽毛分析显示粗蛋白含量为88.83%。在90℃、300分钟后,使用硫酸、1摩尔酸浓度和50克/升固液比时,肽产量最高达到676毫克/克。提出了蛋白质聚集和分解为多肽和氨基酸的反应方案,并建立了肽生产的动力学模型。所提出的动力学模型被证明与实验数据拟合良好,相关系数R = 0.99。

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