Ben Hamad Bouhamed Sana, Kechaou Nabil
Groupe de recherche en Génie des Procédés Agroalimentaires, Laboratoire de recherche en Mécanique des Fluides Appliquée - Génie des Procédés - Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, B.P. 1173, 3038, Sfax, Tunisia.
Bioprocess Biosyst Eng. 2017 May;40(5):715-721. doi: 10.1007/s00449-017-1737-7. Epub 2017 Feb 28.
Poultry feather keratin is the most important by-product from the poultry industry due to its abundance. Different methods have been still applied to process this by-product such as enzymatic hydrolysis which is expensive and inapplicable at the industrial level. This paper presents a study of acid hydrolysis of poultry feathers using different types of acids, sulphuric acid concentration, different temperatures and solid to liquid ratio to obtain a liquid product rich in peptides. The feathers analysis revealed a crude protein content of 88.83%. A maximum peptides production of 676 mg/g was reached using sulphuric acid, 1 molar acid concentration and 50 g/l solid to liquid ratio at a temperature of 90 °C after 300 min. A reaction scheme for protein aggregation and decomposition to polypeptides and amino acids was proposed and a kinetic model for peptides production was developed. The proposed kinetic model proved to be well adapted to the experimental data with R = 0.99.
家禽羽毛角蛋白因其产量丰富,是家禽行业最重要的副产品。仍有不同方法用于处理该副产品,如酶水解,但酶水解成本高昂且不适用于工业规模。本文研究了使用不同类型的酸、硫酸浓度、不同温度和固液比进行家禽羽毛酸水解,以获得富含肽的液体产物。羽毛分析显示粗蛋白含量为88.83%。在90℃、300分钟后,使用硫酸、1摩尔酸浓度和50克/升固液比时,肽产量最高达到676毫克/克。提出了蛋白质聚集和分解为多肽和氨基酸的反应方案,并建立了肽生产的动力学模型。所提出的动力学模型被证明与实验数据拟合良好,相关系数R = 0.99。