Tierney Matthew, Sacco Alessandra
Graduate School of Biomedical Sciences, Sanford-Burnham-Prebys Medical Discovery Institute, 10901 N Torrey Pines Road, La Jolla, CA, 92037, USA.
Development, Aging and Regeneration Program, Sanford Children's Health Research Center, Sanford-Burnham-Prebys Medical Discovery Institute, 10901 N Torrey Pines Rd, La Jolla, CA, 92037, USA.
Methods Mol Biol. 2017;1556:223-236. doi: 10.1007/978-1-4939-6771-1_11.
Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.
骨骼肌干细胞（MuSC）的分离与移植是评估其自我更新和组织修复能力的重要工具。近年来显著的技术进步促使了这些方法的优化，提高了我们评估MuSC再生潜力的能力。在此，我们描述基于荧光激活细胞分选（FACS）分离MuSC、进行肌肉内移植以及分析其在宿主组织中植入情况的操作步骤。
