Qu Tao, Zheng Ping, Yang Chengwei, Lan Xu, Zhang Tao, Liu Hua, Wang Shiyong
Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2016 May 25;45(6):587-591. doi: 10.3785/j.issn.1008-9292.2016.11.05.
To investigate the effects of Danshensu on bone formation in ovariectomized rats. Thirty female SD rats were randomly divided into three groups with 10 rats in each:blank control group, model control group and Danshensu group. The osteoporosis model was induced by bilateral ovariectomy and rats in Danshensu group were fed with Danshensu 12.5 mg·kg·d by gavage after ostroporosis model induced. All animals were sacrificed after 90 days. The bone mineral density (BMD) of the whole body, femur and lumbar vertebra was measured by dual energy X-ray absorptiometry. The biomechanical properties of femur were measured by AG-IS mechanical universal testing machine. Serum osteocalcin and bone alkaline phosphates (BALP) levels were measured by ELISA. The number of osteoblasts of proximal femoral metaphysis was counted with light microscopy after HE staining. Compared with blank control group, BMD, biomechanical properties of femur, serum osteocalcin and BALP levels and the number of osteoblasts were decreased in model control group (<0.05 or <0.01). While compared with model control group, BMDs of the whole body, femur and lumbar vertebra, the elastic modulus, maximum load, yield strength, breaking point load of femur, the serum levels of osteocalcin and BALP, and the number of osteoblasts were significantly improved in Danshensu group (<0.05 or <0.01). Danshensu can improve bone quality by increasing bone density, improving biomechanical properties, promoting the expression of osteogenesis-related factors, and increasing the number of osteoblasts.
探讨丹参素对去卵巢大鼠骨形成的影响。将30只雌性SD大鼠随机分为三组，每组10只：空白对照组、模型对照组和丹参素组。通过双侧卵巢切除术诱导骨质疏松模型，丹参素组大鼠在诱导骨质疏松模型后，通过灌胃给予丹参素12.5mg·kg·d。90天后处死所有动物。采用双能X线吸收法测量全身、股骨和腰椎的骨密度（BMD）。用AG-IS力学万能试验机测量股骨的生物力学性能。采用酶联免疫吸附测定法（ELISA）检测血清骨钙素和骨碱性磷酸酶（BALP）水平。HE染色后，用光镜计数股骨近端干骺端成骨细胞数量。与空白对照组相比，模型对照组的BMD、股骨生物力学性能、血清骨钙素和BALP水平以及成骨细胞数量均降低（<0.05或<0.01）。与模型对照组相比，丹参素组全身、股骨和腰椎的BMD、股骨的弹性模量、最大载荷、屈服强度、断裂点载荷、血清骨钙素和BALP水平以及成骨细胞数量均显著改善（<0.05或<0.01）。丹参素可通过增加骨密度、改善生物力学性能、促进成骨相关因子表达及增加成骨细胞数量来改善骨质量。
