Gao Yuhai, Yang Fangfang, Xi Huirong, Li Wenyuan, Zhen Ping, Chen Keming
Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2016 May 25;45(6):581-586. doi: 10.3785/j.issn.1008-9292.2016.11.04.
To investigate the effect of icariin total flavonoids capsules (ITFC) on bone mineral density (BMD) and bone histomorphometry in growing rats and its anti-osteoporosis mechanism. Thirty female SD rats were randomly divided into 3 groups:normal control group, ITFC-1 group and ITFC-2 group. Rats in ITFC-1 group and ITFC-2 group were fed with 50 mg·kg·d or 100 mg·kg·d ITFC, respectively, and those in normal control group were fed with equal volume of distilled water. The whole body BMD was measured after 4, 8 and 12 weeks, and BMDs of the right femur and lumbar vertebrae were measured after 12 weeks. The serum levels of tartaric acid phosphatase 5b (TRACP 5b) and bone alkaline phosphatase (BALP) were measured by ELISA. Bone morphometry was performed on the right tibia. There were no significant differences in the body weight increase between normal control group and two ITFC groups (all >0.05). There were also no significant differences in whole body BMDs after 4 and 8 weeks between normal control group and ITFC groups (all >0.05). After 12 weeks, the whole body BMD, BMD of bone , serum BALP level and trabecular area in ITFC-1 group and ITFC-2 group were significantly higher, trabecular separation was significantly lower than that in normal control group (all <0.05); and the trabecular width and the number in ITFC-2 group were also significantly higher, and serum TRACP 5b level was significantly lower than that in normal control group (all <0.05). The BMD of bone , serum BALP level, trabecular number and area in ITFC-2 group were significantly higher, and serum TRACP 5b level was significantly lower than that in ITFC-1 group (all <0.05). ITFC can prevent osteoporosis by increasing bone density and bone formation, decreasing bone resorption and improving microstructure of bone.
探讨淫羊藿总黄酮胶囊（ITFC）对生长发育期大鼠骨密度（BMD）、骨组织形态计量学的影响及其抗骨质疏松机制。将30只雌性SD大鼠随机分为3组：正常对照组、ITFC-1组和ITFC-2组。ITFC-1组和ITFC-2组大鼠分别给予50mg·kg·d或100mg·kg·d的ITFC，正常对照组大鼠给予等体积蒸馏水。分别于4周、8周和12周后测量大鼠全身骨密度，12周后测量右侧股骨和腰椎骨密度。采用酶联免疫吸附测定法（ELISA）检测血清抗酒石酸酸性磷酸酶5b（TRACP 5b）和骨碱性磷酸酶（BALP）水平。对右侧胫骨进行骨形态计量学分析。正常对照组与两个ITFC组大鼠体重增加量比较，差异均无统计学意义（均>0.05）。正常对照组与ITFC组大鼠4周和8周后全身骨密度比较，差异均无统计学意义（均>0.05）。12周后，ITFC-1组和ITFC-2组大鼠全身骨密度、骨骨密度、血清BALP水平和骨小梁面积均显著高于正常对照组，骨小梁间距显著低于正常对照组（均<0.05）；ITFC-2组骨小梁宽度和数量也显著高于正常对照组，血清TRACP 5b水平显著低于正常对照组（均<0.05）。ITFC-2组骨骨密度、血清BALP水平、骨小梁数量和面积均显著高于ITFC-1组，血清TRACP 5b水平显著低于ITFC-1组（均<0.05）。ITFC可通过增加骨密度和骨形成、减少骨吸收及改善骨微结构来预防骨质疏松。
