School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Methods Appl Fluoresc. 2017 Mar 1;5(1):015004. doi: 10.1088/2050-6120/aa5d2c.
We demonstrate a method to maximise the fluorescence enhancement from a dye using gold coated diffraction gratings. Rotations about the azimuth provides a convenient approach to maximise the coupling between the grating and excitation source while achieving enhancements comparable to traditional optical configurations where the grating and in plane light vectors are parallel. This approach yields a 30 fold enhancement in the fluorescence signal over metal free substrates, while opening up the range of possible orientations and configurations suitable for fluorescence enhancement applications.
我们展示了一种利用金覆盖的衍射光栅最大限度地提高染料荧光增强的方法。绕方位角的旋转提供了一种方便的方法来最大限度地耦合光栅和激发源，同时实现与传统光学配置相当的增强，其中光栅和平行于平面的光矢量是平行的。这种方法使荧光信号相对于无金属基底增强了 30 倍，同时为适合荧光增强应用的可能取向和配置打开了范围。
