猪痢疾短螺旋体血清分型的更新

An update of Brachyspira hyodysenteriae serotyping.

作者信息

Herbst Werner, Schneider Simone, Baljer Georg, Barth Stefanie A

机构信息

Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Gießen, Frankfurter Str. 85-89, 35392 Gießen, Germany.

出版信息

Res Vet Sci. 2017 Apr;111:135-139. doi: 10.1016/j.rvsc.2017.02.015. Epub 2017 Feb 16.

DOI:10.1016/j.rvsc.2017.02.015

PMID:28249175

Abstract

Brachyspira (B.) hyodysenteriae the causative agent of swine dysentery (SD) has been divided into 9 serotypes on basis of its lipooligosaccharide (LOS). Knowledge on circulating serotypes in Europe, however, is rare. Regarding that immunity to SD is serotype specific an update of B. hyodysenteriae serotyping was undertaken. A LOS band of 10 to 25kDa was identified being appropriate for this purpose. Isolates from Germany, Spain, Denmark, USA and Japan were characterized in the immunoblot by sera raised to serotypes 1 through 7, serogroups H and I (reference strains) and to eight German strains. In total, 57 (51%) isolates responded to at least one of the antisera. Regarding German isolates (n=75) only 35 (46.7%) were identified but mainly by antisera to German strains. Positive Spanish isolates (12 of 17) yielded similar results. In contrast, positively reacting Danish isolates (9 of 12) were mainly identified by antisera to the reference strains as it was the case for recent U.S. (1 of 8) and Japanese isolates (3 of 5). Results indicate that B. hyodysenteriae has a high degree of serological heterogeneity that has probably differently developed in diverse geographical areas over time. This situation represents a challenge for vaccine development.

摘要

猪痢疾密螺旋体（Brachyspira (B.) hyodysenteriae）是猪痢疾（SD）的病原体，根据其脂寡糖（LOS）已被分为9个血清型。然而，关于欧洲流行血清型的了解却很少。鉴于对猪痢疾的免疫力具有血清型特异性，因此对猪痢疾密螺旋体进行了血清分型更新。已确定10至25kDa的LOS条带适用于此目的。通过针对1至7血清型、H和I血清群（参考菌株）以及8株德国菌株产生的血清，对来自德国、西班牙、丹麦、美国和日本的分离株进行了免疫印迹鉴定。总共57株（51%）分离株对至少一种抗血清有反应。对于德国分离株（n = 75），仅鉴定出35株（46.7%），但主要是通过针对德国菌株的抗血清鉴定的。西班牙阳性分离株（17株中的12株）产生了类似的结果。相比之下，丹麦阳性分离株（12株中的9株）主要是通过针对参考菌株的抗血清鉴定的，美国近期分离株（8株中的1株）和日本分离株（5株中的3株）也是如此。结果表明，猪痢疾密螺旋体具有高度的血清学异质性，随着时间的推移，这种异质性可能在不同地理区域以不同方式发展。这种情况对疫苗开发构成了挑战。