Wang Zhan-di, Feng Ying, Ma Li-Yi, Li Xian, Ding Wei-Feng, Chen Xiao-Ming
Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
Research Institute of Resource Insects of the Chinese Academy of Forestry, Kunming 650233, Yunnan, China; The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650233, Yunnan, China.
Biomed Pharmacother. 2017 May;89:438-446. doi: 10.1016/j.biopha.2017.02.036. Epub 2017 Feb 27.
White wax (WW) has been traditionally used to treat hair loss in China. However there has been no reporter WW and its extract responsible for hair growth-promoting effect on androgenetic alopecia. In this paper, we examined the hair growth-promoting effects of WW and policosanol of white wax (WWP) on model animal of androgenetic alopecia and the potential target cell of WW and WWP. WW (1, 10 and 20%) and WWP (0.5, 1 and 2%) were applied topically to the backs of mice. Finasteride (2%) was applied topically as a positive control. MTS assays were performed to evaluate cell proliferation in culture human follicle dermal papilla cells (HFDPCs). The inhibition of WW and WWP for 5α- reductase were tested in Vitro. Results showed more lost hairs were clearly seen in mice treated with TP only and TP plus vehicle. Mice which received TP plus WW and WWP showed less hair loss. WW and WWP showed an outstanding hair growth-promoting activity as reflected by the follicular length, follicular density, A/T ratio, and hair bulb diameter. The optimal treatment effect was observed at 10% WW and 1% WWP, which were better than 2% finasteride treatment. MTS assay results suggested that WW and WWP remarkably increased the proliferation of HFDPCs. Inhibitor assay of 5α- reductase showed that WW and WWP inhibited significantly the conversion of testosterone to dihydrotesterone, and the IC values of WW and WWP were higher than that of finasteride. In Conclusion, WW and WWP could act against testosterone-induced alopecia in mice, and they promoted hair growth by inhibiting 5α-reductase activity and HFDPCs proliferation. DPCs is the target cell of WW and WWP.
在中国，白蜡传统上一直被用于治疗脱发。然而，尚无报道表明白蜡及其提取物对雄激素性脱发有促进头发生长的作用。在本文中，我们研究了白蜡（WW）和白蜡中的 policosanol（WWP）对雄激素性脱发模型动物的促头发生长作用以及WW和WWP的潜在靶细胞。将WW（1%、10%和20%）和WWP（0.5%、1%和2%）局部涂抹于小鼠背部。将非那雄胺（2%）局部涂抹作为阳性对照。进行MTS试验以评估培养的人毛囊真皮乳头细胞（HFDPCs）的细胞增殖。在体外测试WW和WWP对5α-还原酶的抑制作用。结果显示，仅用睾酮（TP）和TP加赋形剂处理的小鼠明显可见更多脱发。接受TP加WW和WWP的小鼠脱发较少。从毛囊长度、毛囊密度、A/T比和毛球直径可以看出，WW和WWP表现出显著的促头发生长活性。在10%的WW和1%的WWP时观察到最佳治疗效果，优于2%非那雄胺治疗。MTS试验结果表明，WW和WWP显著增加了HFDPCs的增殖。5α-还原酶抑制试验表明，WW和WWP显著抑制睾酮向二氢睾酮的转化，且WW和WWP的IC值高于非那雄胺。总之，WW和WWP可以对抗睾酮诱导的小鼠脱发，它们通过抑制5α-还原酶活性和HFDPCs增殖来促进头发生长。真皮乳头细胞（DPCs）是WW和WWP的靶细胞。
