Sweatman W J, Brandon D R, Cranstone S, Gooderham R, Walker J R
Lilly Research Center Limited, Eli Lilly & Co., Surrey, United Kingdom.
J Pharmacol Methods. 1987 Nov;18(3):227-37. doi: 10.1016/0160-5402(87)90073-8.
The preparation of indium-111 tropolonate-radiolabeled guinea pig peripheral mixed white cells (greater than 80% neutrophils) is described. Autologous rather than homologous cells are required to provide a population of labeled, functional cells on reintroduction to the animals. Surgery has been shown to result in a profound neutropenia from which the animals must recover before removal of blood for cell preparation. The response of radiolabeled cells parallels that of the unlabeled cell population to a chemotaxin, leukotriene B4. This material causes a profound neutropenia of rapid onset accompanied by a parallel fall in blood radioactivity. The fall in circulating radiolabel is accompanied by an increase in radioactivity in the thoracic region. These changes have been monitored externally using an automated isotope monitoring system.
本文描述了铟 - 111 托酚酮标记的豚鼠外周混合白细胞（中性粒细胞大于80%）的制备方法。需要自体而非同源细胞，以便在重新引入动物体内时提供一群有标记的功能性细胞。手术已被证明会导致严重的中性粒细胞减少症，在采集血液进行细胞制备之前，动物必须从中恢复。放射性标记细胞对趋化因子白三烯B4的反应与未标记细胞群体的反应相似。这种物质会迅速引发严重的中性粒细胞减少症，同时血液放射性平行下降。循环放射性标记物的下降伴随着胸部区域放射性的增加。这些变化已使用自动同位素监测系统进行外部监测。
