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冷冻保存的细胞和组织中的单细胞转录组保守性

Single-cell transcriptome conservation in cryopreserved cells and tissues.

作者信息

Guillaumet-Adkins Amy, Rodríguez-Esteban Gustavo, Mereu Elisabetta, Mendez-Lago Maria, Jaitin Diego A, Villanueva Alberto, Vidal August, Martinez-Marti Alex, Felip Enriqueta, Vivancos Ana, Keren-Shaul Hadas, Heath Simon, Gut Marta, Amit Ido, Gut Ivo, Heyn Holger

机构信息

CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.

Universitat Pompeu Fabra (UPF), Barcelona, Spain.

出版信息

Genome Biol. 2017 Mar 1;18(1):45. doi: 10.1186/s13059-017-1171-9.

DOI:10.1186/s13059-017-1171-9
PMID:28249587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5333448/
Abstract

A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.

摘要

已经描述了多种单细胞RNA制备方法。到目前为止，方案都需要新鲜材料，这阻碍了复杂的研究设计。我们描述了一种样本保存方法，该方法能在活的单细胞中维持转录本，使采样时间和地点与后续处理步骤分离。我们使用3'端和全长RNA制备方法对1000多个新鲜和冷冻保存的细胞进行了单细胞转录组测序。我们的结果证实，保存过程不会改变转录谱。这大大拓宽了单细胞转录组学的应用范围，并可能导致未来研究设计的范式转变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/f3b5a957d4bd/13059_2017_1171_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/eec21501b2bb/13059_2017_1171_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/1ccb51d77947/13059_2017_1171_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/7106e606fb91/13059_2017_1171_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/b5cdebcd30d2/13059_2017_1171_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/21522043ddb5/13059_2017_1171_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/4bb3d7f480b5/13059_2017_1171_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/f3b5a957d4bd/13059_2017_1171_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/eec21501b2bb/13059_2017_1171_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/1ccb51d77947/13059_2017_1171_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/7106e606fb91/13059_2017_1171_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/b5cdebcd30d2/13059_2017_1171_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/21522043ddb5/13059_2017_1171_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/4bb3d7f480b5/13059_2017_1171_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7fe/5333448/f3b5a957d4bd/13059_2017_1171_Fig7_HTML.jpg

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