Bardell D
Department of Biology, Kean College of New Jersey, Union 07083.
Microbios. 1987;51(208-209):151-7.
A 1.0 ml suspension of herpes simplex virus type 1 in tissue culture growth medium was exposed at 37 degrees C to eight puffs of smoke from one cigarette. Each puff consisted of 25.0 ml of mainstream smoke, and was delivered by a mechanical smoking apparatus. At 0, 15 and 60 min after exposure the virus was assayed for infectivity using HEp-2 cells as the host. Neither filter nor non-filter cigarette smoke affected the infectivity of the virus, or the viability of HEp-2 cells treated with smoke in a manner similar to the virus. The filter cigarette contained 19.0 mg of tar and 1.2 mg of nicotine, and the non-filter cigarette had 23.0 mg of tar and 1.4 mg of nicotine. Smoke from four non-filter cigarettes delivered over a 4 h period caused a 4-log10 drop in virus infectivity titre, and smoke from four filter cigarettes caused more than a 1-log10 drop in titre. Smoke from four non-filter or filter cigarettes was highly cytocidal for HEp-2 cells.
将1.0毫升1型单纯疱疹病毒在组织培养生长培养基中的悬浮液，于37摄氏度下暴露于一支香烟的八口烟雾中。每口烟雾由25.0毫升主流烟雾组成，通过机械吸烟装置输送。在暴露后0、15和60分钟，以人喉表皮样癌细胞（HEp-2细胞）作为宿主对病毒的感染性进行测定。过滤嘴香烟烟雾和非过滤嘴香烟烟雾均未影响病毒的感染性，也未影响以与病毒类似方式用烟雾处理的HEp-2细胞的活力。过滤嘴香烟含有19.0毫克焦油和1.2毫克尼古丁，非过滤嘴香烟含有23.0毫克焦油和1.4毫克尼古丁。在4小时内输送的四支非过滤嘴香烟的烟雾使病毒感染性滴度下降了4个对数级，四支过滤嘴香烟的烟雾使滴度下降超过1个对数级。四支非过滤嘴或过滤嘴香烟的烟雾对HEp-2细胞具有高度细胞毒性。
