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小鼠线粒体DNA与核DNA比例分析

Analysis of mtDNA/nDNA Ratio in Mice.

作者信息

Quiros Pedro M, Goyal Aashima, Jha Pooja, Auwerx Johan

机构信息

Laboratory for Integrative and Systems Physiology, École Polytechnique Federale de Lausanne, Lausanne, Switzerland.

出版信息

Curr Protoc Mouse Biol. 2017 Mar 2;7(1):47-54. doi: 10.1002/cpmo.21.

DOI:10.1002/cpmo.21
PMID:28252199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5335900/
Abstract

Mitochondrial DNA (mtDNA) lacks the protection provided by the nucleosomes in the nuclear DNA and does not have a DNA repair mechanism, making it highly susceptible to damage, which can lead to mtDNA depletion. mtDNA depletion compromises the efficient function of cells and tissues and thus impacts negatively on health. Here, we describe a brief and easy protocol to quantify mtDNA copy number by determining the mtDNA/nDNA ratio. The procedure has been validated using a cohort of young and aged mice. © 2017 by John Wiley & Sons, Inc.

摘要

线粒体DNA（mtDNA）缺乏核DNA中核小体提供的保护，且没有DNA修复机制，这使其极易受到损伤，进而可能导致mtDNA耗竭。mtDNA耗竭会损害细胞和组织的有效功能，从而对健康产生负面影响。在此，我们描述了一种通过测定mtDNA与核DNA（nDNA）的比例来量化mtDNA拷贝数的简便方法。该方法已在一组年轻和老年小鼠中得到验证。© 2017约翰威立父子公司版权所有

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d27/5335900/f316a561dbae/nihms830312f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d27/5335900/5fefd1436965/nihms830312f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d27/5335900/f316a561dbae/nihms830312f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d27/5335900/5fefd1436965/nihms830312f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d27/5335900/f316a561dbae/nihms830312f2.jpg

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Inefficient DNA Repair Is an Aging-Related Modifier of Parkinson's Disease.低效的DNA修复是帕金森病的一种与衰老相关的修饰因素。
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Mol Neurobiol. 2025 Jul 31. doi: 10.1007/s12035-025-05213-9.
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