Cheeseman Michael T, Hood Derek W
Developmental Biology Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh, Easter Bush, United Kingdom.
Molecular Genetics Unit, MRC Harwell Institute, Harwell Science and Innovation Campus, Oxfordshire, United Kingdom.
Curr Protoc Mouse Biol. 2017 Mar 2;7(1):29-46. doi: 10.1002/cpmo.24.
Acute otitis media, inflammation of the middle ear bulla, is the most common bacterial infection in children. For one of the principal otopathogens, non-typeable Haemophilus influenzae (NTHi), animal models allow us to investigate host-microbial interactions relevant to the onset and progression of infection and to study treatment of middle ear disease. We have established a robust model of NTHi middle ear infection in the Junbo mouse. Intranasal inoculation with NTHi produces high rates of bulla infection and high bacterial titers in bulla fluids; bacteria can also spread down the respiratory tract to the mouse lung. An innate immune response is detected in the bulla of Junbo mice following NTHi infection, and bacteria are maintained in some ears at least up to day 56 post-inoculation. The Junbo/NTHi infection model facilitates studies on bacterial pathogenesis and antimicrobial intervention regimens and vaccines for better treatment and prevention of NTHi middle ear infection. © 2017 by John Wiley & Sons, Inc.
急性中耳炎，即中耳鼓室的炎症，是儿童最常见的细菌感染。对于主要的耳病原体之一——不可分型流感嗜血杆菌（NTHi），动物模型使我们能够研究与感染的发生和进展相关的宿主-微生物相互作用，并研究中耳疾病的治疗方法。我们已经在君博小鼠中建立了一个强大的NTHi中耳感染模型。经鼻接种NTHi可产生较高的鼓室感染率和鼓室液中较高的细菌滴度；细菌还可沿呼吸道向下扩散至小鼠肺部。在NTHi感染后的君博小鼠鼓室中可检测到先天性免疫反应，并且在接种后至少56天内，一些耳朵中仍存在细菌。君博/NTHi感染模型有助于研究细菌发病机制以及抗菌干预方案和疫苗，以更好地治疗和预防NTHi中耳感染。© 2017约翰威立国际出版公司
