Bjørnerheim R, Simonsen S, Golf S
Institute of Pathology, University of Oslo, Norway.
Scand J Clin Lab Invest. 1987 Nov;47(7):661-5.
A transplanted (Tx) heart will probably compensate its sympathetic denervation by increasing the sensitivity to adrenergic stimulants. To evaluate whether this also engages the myocardial adenylate cyclase (AC) system, small endomyocardial biopsies were collected at right heart catheterization both from patients with heart Tx with no signs of rejection and from a group of patients serving as controls. In crude homogenates from these biopsies, AC activity was measured at basal conditions and following in vitro stimulation with optimal concentrations of beta-receptor agonists, histamine and sodium fluoride (NaF). The Tx group exhibited a 75% (p = 0.01) higher activation of AC with isoproterenol, and a 62% (p less than 0.05) higher AC activation with terbutaline compared with the control group. The differences in AC activation by histamine and NaF were insignificant. Thus, sympathetic denervation leads to increased activation of the beta-adrenoceptor-coupled AC system, leaving the histaminergic system unaltered. The upregulation does probably engage the receptor part of the receptor-AC system exclusively, since no elevation of NaF stimulation of AC was found in the Tx hearts.
移植心脏可能会通过增加对肾上腺素能刺激物的敏感性来补偿其交感神经去神经支配。为了评估这是否也涉及心肌腺苷酸环化酶（AC）系统，在右心导管检查时，从无排斥反应迹象的心脏移植患者以及一组作为对照的患者中采集了小的心内膜活检组织。在这些活检组织的粗匀浆中，在基础条件下以及用最佳浓度的β受体激动剂、组胺和氟化钠（NaF）进行体外刺激后测量AC活性。与对照组相比，移植组在用异丙肾上腺素刺激时AC的激活增加了75%（p = 0.01），在用特布他林刺激时AC的激活增加了62%（p < 0.05）。组胺和NaF对AC激活的差异不显著。因此，交感神经去神经支配导致β肾上腺素能受体偶联的AC系统激活增加，而组胺能系统未改变。这种上调可能仅涉及受体-AC系统的受体部分，因为在移植心脏中未发现NaF刺激AC的升高。
