Omary M B, Kagnoff M F
Department of Medicine, University of California, San Diego, La Jolla 92093.
Science. 1987 Dec 11;238(4833):1578-81. doi: 10.1126/science.2825352.
Vasoactive intestinal peptide (VIP) is a neuropeptide with broad tissue distribution. Although its precise function is unknown, it is thought to exert its effect, at least in part, by interacting with cell surface receptors. Nuclear receptors for VIP have now been identified by specific binding of 125I-labeled VIP to nuclei of a human colonic adenocarcinoma cell line (HT29) and by cross-linking of 125I-labeled VIP to its receptor on intact nuclei. In contrast, 125I-labeled transferrin shows only background binding to nuclei but significant binding to intact cells. Purity of the isolated nuclei was further substantiated by electron microscopy. The apparent molecular sizes of the VIP--cross-linked nuclear and cell surface receptors are similar but not identical.
血管活性肠肽（VIP）是一种在组织中广泛分布的神经肽。尽管其确切功能尚不清楚，但人们认为它至少部分地通过与细胞表面受体相互作用来发挥作用。通过将125I标记的VIP与人类结肠腺癌细胞系（HT29）的细胞核进行特异性结合，以及将125I标记的VIP与完整细胞核上的受体进行交联，现已鉴定出VIP的核受体。相比之下，125I标记的转铁蛋白仅与细胞核有背景结合，但与完整细胞有显著结合。通过电子显微镜进一步证实了分离细胞核的纯度。VIP交联的核受体和细胞表面受体的表观分子大小相似但不相同。
