Zhang Lemeng, Dong Xiaocui, Wang Chonggang, Zuo Zhenghong, Chen Meng
Key Laboratory of Ministry of Education for Subtropical Wetland Ecosystem Research, Xiamen University, Xiamen 361102, China.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China.
J Environ Sci (China). 2017 Feb;52:98-104. doi: 10.1016/j.jes.2016.03.011. Epub 2016 Apr 18.
This study was conducted to assess the effects of difenoconazole (DFZ), a triazole fungicide, on the hepatic biotransformation system and its bioaccumulation in marine medaka (Oryzias melastigma). Fish were exposed to DFZ (1, 10, 100, 1000ng/L) for 180days. The results showed that: (1) The mRNA levels of hepatic CYP1A1, CYP1B, CYP1C1, CYP27B and CYP3A40 were up-regulated, but those of CYP3A38 and CYP27A1 were down-regulated. (2) The activity of ethoxyresorufin-O-deethylase (EROD) and the content of reduced glutathione (GSH) in the liver were increased in the DFZ-treated groups, and glutathione S-transferase (GST) activity was increased in the 100 and 1000ng/L groups. (3) DFZ was accumulated in the muscle and the biological concentration factors in the 10, 100, and 1000ng/L groups were respectively 149, 81 and 25. These results suggested that long-term exposure to DFZ at low concentrations would result in a bioaccumulation of this compound and disturb the biotransformation system.
本研究旨在评估三唑类杀菌剂苯醚甲环唑（DFZ）对海洋青鳉（Oryzias melastigma）肝脏生物转化系统及其生物累积的影响。将鱼类暴露于DFZ（1、10、100、1000纳克/升）中180天。结果表明：（1）肝脏CYP1A1、CYP1B、CYP1C1、CYP27B和CYP3A40的mRNA水平上调，但CYP3A38和CYP27A1的mRNA水平下调。（2）DFZ处理组肝脏中乙氧异吩恶唑-O-脱乙基酶（EROD）的活性和还原型谷胱甘肽（GSH）的含量增加，100和1000纳克/升组谷胱甘肽S-转移酶（GST）活性增加。（3）DFZ在肌肉中蓄积，10、100和1000纳克/升组的生物浓缩系数分别为149、81和25。这些结果表明，长期低浓度暴露于DFZ会导致该化合物的生物累积并扰乱生物转化系统。
