Klenova A V, Borodina N P, Shevliagin V Ia
Vopr Onkol. 1987;33(11):57-61.
Virus reproduction and activation were studied in human cells transformed with human polyoma virus. At the stage of spontaneous viral production, such methods of activation as somatic hybridization and DNA transfection were successful. Special methods of viral activation (treatment with mitomycin C and DEAE--dextran, and subsequent homologous DNA transfection) were required when viral production by cells ceased completely.
在用人多瘤病毒转化的人类细胞中研究了病毒的繁殖和激活。在自发病毒产生阶段,体细胞杂交和DNA转染等激活方法是成功的。当细胞的病毒产生完全停止时,需要特殊的病毒激活方法(用丝裂霉素C和DEAE-葡聚糖处理,随后进行同源DNA转染)。