多形钩苞菊二氯甲烷和丁醇部位对小鼠母体生殖结局、胚胎-胎儿发育及DNA完整性的影响
Effects of dichloromethane and butanol fractions of Gochnatia polymorpha floccosa in maternal reproductive outcome, embryo-fetal development and DNA integrity in mice.
作者信息
Pessatto Lucas Roberto, Auharek Sarah Alves, Gonçalves Caroline Amélia, de David Natan, Monreal Antônio Carlos Duenhas, Kassuya Cândida Aparecida Leite, Antoniolli-Silva Andréia Conceição Milan Brochado, Stefanello Maria Élida Alves, Oliveira Rodrigo Juliano
机构信息
Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica (CeTroGen), Hospital Universitário Maria Aparecida Pedrossian (HUMAP), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil; Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde (CCBS), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil.
Faculdade de Medicina do Mucuri (FAMMUC), Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Teófilo Otoni, Minas Gerais, Brazil.
出版信息
J Ethnopharmacol. 2017 Mar 22;200:205-208. doi: 10.1016/j.jep.2017.02.042. Epub 2017 Feb 27.
ETHNOPHARMACOLOGICAL RELEVANCE
Gochnatia polymorpha ssp. floccosa (Asteraceae) also known as ''Cambará'' is used as medicinal plant in Brazil to treat infections and inflammation. Previous studies showed that its ethanolic extract could be bioprospecting of a new anti-inflammatory phytotherapy for use during pregnancy. This work aimed to evaluate dichloromethane (DCM) and butanolic (BT) fractions from G. polymorpha on embryo-fetal development and DNA integrity in mice.
MATERIALS AND METHODS
Female mice were treated with 50 and 20mg/kg of the DCM and BT fractions, respectively, during organogenesis and gestational period.
RESULTS AND CONCLUSION
The present study shows that DCM and BT fractions from G. polymorpha possess mutagenic activity but are not teratogenic. Based on the fact that the reproductive indices are similar in control and treated animals, we may infer that the mutagenic effect was in somatic cell, at least in part, because the reabsorption number and reabsorption rates did not change in DCM and BT exposed groups.
民族药理学相关性
多形钩苞菊亚种絮状钩苞菊（菊科），也被称为“坎巴拉”，在巴西被用作药用植物来治疗感染和炎症。先前的研究表明，其乙醇提取物可能是一种可用于孕期的新型抗炎植物疗法的生物勘探对象。这项工作旨在评估多形钩苞菊的二氯甲烷（DCM）和丁醇（BT）组分对小鼠胚胎-胎儿发育和DNA完整性的影响。
材料与方法
在器官形成期和妊娠期，分别用50mg/kg和20mg/kg的DCM和BT组分处理雌性小鼠。
结果与结论
本研究表明，多形钩苞菊的DCM和BT组分具有诱变活性，但不具有致畸性。基于对照动物和处理动物的生殖指标相似这一事实，我们可以推断，诱变作用至少部分发生在体细胞中，因为DCM和BT暴露组的重吸收数量和重吸收率没有变化。
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