Jurczyszyn Kamil, Woźniak Marta, Symonowicz Krzysztof, Sprutta Natasza, Latos-Grażyński Lechosław, Ziółkowski Piotr, Ivonyak Yurij, Tuchowski Piotr, Trzeciakowski Witold
Department of Oral Surgery, Wrocław Medical University, 26 Krakowska, 50-425 Wrocław, Poland.
Department of Pathology, Wrocław Medical University, 1 Marcinkowskiego, 50-367, Wrocław, Poland.
Photodiagnosis Photodyn Ther. 2017 Jun;18:179-184. doi: 10.1016/j.pdpdt.2017.02.014. Epub 2017 Feb 27.
The search for new photosensitizers for application in photodynamic therapy has quite a long history. In the past, a large number of potent photosensitizers were used in both basic and clinical studies; however, only a few turned out to be effective and safe.
In the present study, two compounds were used: 5-aminolevulinic acid in two formulations (free and liposomal), and the newly synthesized porphyrin, 5,10,15,20-tetra-p-tolyl-22,24-dithiadibenzocarbaporphyrin, termed DTDB. Two different light sources, a halogen lamp (wavelength 450+/-20nm) and a diode laser (wavelength 450nm), were used to sensitize the compounds. The entire experiment was performed on mice bearing mouse mammary carcinoma, 4T1.
The results showed that the DTDB-PDT applied by means of a laser proved to be most effective and caused the 83.3% necrosis of treated tumors. The overall effect of laser PDT was more potent than that of the halogen lamp-mediated PDT.
In the present study, we would like to show that modifications of porphyrins lead to an increase in the effectiveness of PDT and that this effect could also be potentiated by using a proper light source.
寻找用于光动力疗法的新型光敏剂已有很长历史。过去，大量强效光敏剂被用于基础和临床研究；然而，只有少数被证明是有效且安全的。
在本研究中，使用了两种化合物：两种制剂形式的5-氨基乙酰丙酸（游离型和脂质体型），以及新合成的卟啉5,10,15,20-四对甲苯基-22,24-二硫杂二苯并咔卟啉，称为DTDB。使用两种不同的光源，卤素灯（波长450±20nm）和二极管激光器（波长450nm）来使化合物致敏。整个实验在携带小鼠乳腺癌4T1的小鼠身上进行。
结果表明，通过激光应用的DTDB光动力疗法被证明是最有效的，导致治疗肿瘤出现83.3%的坏死。激光光动力疗法的总体效果比卤素灯介导的光动力疗法更强。
在本研究中，我们想要表明卟啉的修饰会导致光动力疗法有效性的提高，并且通过使用合适的光源这种效果还可以增强。
