Tedesco Pietro, Di Schiavi Elia, Esposito Fortunato Palma, de Pascale Donatella
Institute of Protein Biochemistry, National Research Council, Naples, Italy.
Institute of Bioscience and Bioresources, National Research Council, Naples, Italy.
Bio Protoc. 2016 Oct 20;6(20). doi: 10.21769/BioProtoc.1964.
This protocol describes two biological assays to evaluate pathogenicity of complex (Bcc) strains against the nematode Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.
本方案描述了两种生物学测定方法，用于评估复杂（Bcc）菌株对线虫的致病性。具体而言，这两种测定方法使人们能够确定所研究的Bcc菌株是否能够通过肠道定殖（慢杀测定法，SKA）或通过产生毒素（快杀测定法，FKA）来杀死线虫。这两种测定方法的主要区别在于对线虫的不同杀伤动力学。
