兔睾丸溶酶体的部分纯化及特性
Partial purification and properties of lysosomes from rabbit testes.
作者信息
Ninjoor V, Srivastava P N
机构信息
Department of Biochemistry, University of Georgia, Athens.
出版信息
Andrologia. 1987 Jan-Feb;19(1):80-5. doi: 10.1111/j.1439-0272.1987.tb01864.x.
Rabbit testes lysosomes were prepared by differential centrifugation and density gradient centrifugation on Percoll. The particulate material sedimented at 1.054 g/cm3. The enrichment in the activities of lysosomal marker enzymes was 9-fold. The contamination by other subcellular organelles such as mitochondria, microsomes and the plasma membrane was limited to 0.1% or less. The marker enzymes of isolated lysosomes exhibited bound activity of 80%, thus indicating the integrity of granules. The testicular lysosomes also exhibited osmofragility characteristics.
通过差速离心和在Percoll上进行密度梯度离心制备兔睾丸溶酶体。颗粒物质在1.054 g/cm³ 处沉淀。溶酶体标记酶活性的富集倍数为9倍。线粒体、微粒体和质膜等其他亚细胞器的污染限制在0.1%或更低。分离出的溶酶体的标记酶表现出80%的结合活性，从而表明颗粒的完整性。睾丸溶酶体也表现出渗透脆性特征。
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