Blas-Rus Noelia, Bustos-Morán Eugenio, Sánchez-Madrid Francisco, Martín-Cófreces Noa B
Servicio de Inmunología, Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Instituto Investigación Sanitaria Princesa (IIS-IP), Diego de León 62, 28006, Madrid, Spain.
Vascular PathophysiologyArea, Centro Nacional Investigaciones Cardiovasculares (CNIC), 28029, Madrid, Spain.
Methods Mol Biol. 2017;1584:31-49. doi: 10.1007/978-1-4939-6881-7_3.
The immune synapse (IS) is a specialized structure that enables cell-cell communication between immune cells. As such, it involves direct cell-to-cell contact. It is sustained by cytoskeletal components that allow the intracellular polarization of different organelles and the surface re-organization of signaling and adhesion receptors. The tubulin-based cytoskeleton is a key player in IS formation and signaling. We describe methods to analyze through Western blot and microscopy analysis the polarization to the IS of the centrosome, also known as microtubule-organizing center (MTOC), the dynamics of microtubule growth and polymerization from the MTOC to the IS and the activation of signaling molecules.
免疫突触（IS）是一种特殊结构，可实现免疫细胞间的细胞间通讯。因此，它涉及细胞间的直接接触。它由细胞骨架成分维持，这些成分允许不同细胞器在细胞内极化以及信号和黏附受体在表面重新组织。基于微管蛋白的细胞骨架在免疫突触的形成和信号传导中起关键作用。我们描述了通过蛋白质印迹法和显微镜分析来分析中心体（也称为微管组织中心，MTOC）向免疫突触的极化、微管从MTOC向免疫突触生长和聚合的动力学以及信号分子激活的方法。
