Lee Joung-Hyun, Kam Lance C
Department of Biomedical Engineering, Columbia University, 120 Amsterdam Ave Mail Code 8904, New York, NY, 10027, USA.
Methods Mol Biol. 2017;1584:291-306. doi: 10.1007/978-1-4939-6881-7_17.
The immune synapse has emerged as a compelling example of structural complexity within cell-cell interfaces. This chapter focuses on the use of microcontact printing to isolate and investigate how spatial organization of signaling molecules drives the function of immune cells. In the process detailed here, multiple rounds of microcontact printing are combined to create patterned surfaces that control the relative spatial localization of CD3 and CD28 signaling in T cells, effectively replacing an antigen presenting cell with an engineered surface. A set of approaches used to address key issues of T cell activation are described and discussed.
免疫突触已成为细胞间界面结构复杂性的一个引人注目的例子。本章重点介绍使用微接触印刷来分离和研究信号分子的空间组织如何驱动免疫细胞的功能。在这里详细描述的过程中，多轮微接触印刷被结合起来以创建图案化表面，该表面控制T细胞中CD3和CD28信号的相对空间定位，有效地用工程表面替代抗原呈递细胞。描述并讨论了用于解决T细胞激活关键问题的一组方法。
