白血病抗原呈递细胞驱动的异常免疫突触
Aberrant Immunological Synapses Driven by Leukemic Antigen-Presenting Cells.
作者信息
Lucas Fabienne McClanahan, Gribben John G
机构信息
Department of Haemato-Oncology, Barts Cancer Institute, Queen Mary University of London, 3rd Floor John Vane Science Centre, Charterhouse Square, London, UK.
Comprehensive Cancer Center, The Ohio State University, EC1M 6BQ, Columbus, OH, USA.
出版信息
Methods Mol Biol. 2017;1584:533-544. doi: 10.1007/978-1-4939-6881-7_33.
Aberrant immune synapse formation between antigen-presenting and immune effector cells is a central mediator of immune dysfunction and can be observed across several haematologic malignancies. Here, we describe the cell preparation, conjugation and immune synapse quantification of B and T cells obtained from patients with leukaemia and the adaptions required when using cells from murine models of disease.
抗原呈递细胞与免疫效应细胞之间异常免疫突触的形成是免疫功能障碍的主要介导因素，在多种血液系统恶性肿瘤中均可观察到。在此，我们描述了从白血病患者获得的B细胞和T细胞的细胞制备、结合及免疫突触定量，以及使用疾病小鼠模型的细胞时所需的调整。
Zotero 插件