Zhang Hongkai, Lerner Richard A
Department of Cell and Molecular Biology, The Scripps Research Institute, Mail Code: MB 10, 10550 N. Torrey Pines Rd, La Jolla, CA, 92037, USA.
Methods Mol Biol. 2017;1575:215-222. doi: 10.1007/978-1-4939-6857-2_13.
There is a need for a general reporter system to study the interactions between a ligand and membrane protein in living cells. Here, we demonstrate a method that allows identification of undiscovered ligand partners of a membrane protein in its natural milieu. This assay will be of importance especially for multiple pass transmembrane proteins such as ion channels and GPCRs.
需要一种通用的报告系统来研究活细胞中配体与膜蛋白之间的相互作用。在此，我们展示了一种方法，该方法能够在天然环境中鉴定膜蛋白未被发现的配体伴侣。这种检测方法对于多跨膜蛋白（如离子通道和G蛋白偶联受体）尤为重要。
