Zhang Zhe, Yu Rongbo, Cao Lei
a Department of Geriatrics , Weihai Municipal Hospital , Weihai , China.
b Department of Cardiovascular , Weihai Municipal Hospital , Weihai , China.
Neurol Res. 2017 May;39(5):453-458. doi: 10.1080/01616412.2017.1297906. Epub 2017 Mar 3.
Cerebral ischemia exhibits a multiplicity of pathophysiological mechanisms. Taurine (Tau), an endogenous substance, possesses a number of cytoprotective properties. The aim of the present study was to examine the neuroprotective effect of Tau, through affecting 12/15 lipoxygenase (12/15-LOX) signal pathway in an acute permanent middle cerebral artery occlusion (MCAO) model of rats.
Sprague-Dawley rats were randomly divided into 3 groups (n = 10), namely the sham-operated group, MCAO group and Tau group. Tau was intraperitoneally administrated immediately after cerebral ischemia. At 24 h after MCAO, neurological function score, brain water content and infarct volume were assessed. The expression of 12/15-lipoxygenase (12/15-LOX), p38 mitogen-activated protein kinase (p38 MAPK), and cytosolic phospholipase A2 (cPLA2) was measured by Western blot. Enzyme-linked immunosorbent assay was used to evaluate the inflammatory factors TNF-α, IL-1β and IL-6 in serum.
Compared with MCAO group, taurine significantly improved neurological function and significantly reduced brain water content (p < 0.05) and infarct volume (p < 0.05). Consistent with these indices, the overexpressions of 12/15-LOX, p38 MAPK, cPLA2, tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were significantly decreased in Tau group (p < 0.05).
Taurine protected the brain from damage caused by MCAO; this effect may be through down-regulation of 12/15-LOX, p38 MAPK, and cPLA2.
脑缺血呈现出多种病理生理机制。牛磺酸（Tau）作为一种内源性物质，具有多种细胞保护特性。本研究旨在通过影响大鼠急性永久性大脑中动脉闭塞（MCAO）模型中的12/15脂氧合酶（12/15-LOX）信号通路，来研究牛磺酸的神经保护作用。
将Sprague-Dawley大鼠随机分为3组（n = 10），即假手术组、MCAO组和牛磺酸组。脑缺血后立即腹腔注射牛磺酸。MCAO术后24小时，评估神经功能评分、脑含水量和梗死体积。通过蛋白质免疫印迹法检测12/15-脂氧合酶（12/15-LOX）、p38丝裂原活化蛋白激酶（p38 MAPK）和胞质磷脂酶A2（cPLA2）的表达。采用酶联免疫吸附测定法评估血清中的炎症因子肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）。
与MCAO组相比，牛磺酸显著改善神经功能，显著降低脑含水量（p < 0.05）和梗死体积（p < 0.05）。与这些指标一致，牛磺酸组中12/15-LOX、p38 MAPK、cPLA2、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的过表达显著降低（p < 0.05）。
牛磺酸可保护大脑免受MCAO所致损伤；这种作用可能是通过下调12/15-LOX、p38 MAPK和cPLA2实现的。
