离体大鼠肝细胞中的同源β-肾上腺素能脱敏
Homologous beta-adrenergic desensitization in isolated rat hepatocytes.
作者信息
García-Sáinz J A, Michel B
机构信息
Departamento de Bioenergética, Universidad Nacional Autónoma de México, D.F.
出版信息
Biochem J. 1987 Sep 1;246(2):331-6. doi: 10.1042/bj2460331.
Abstract
Hepatocytes from hypothyroid rats have a marked beta-adrenergic responsiveness. Preincubation of these hepatocytes with isoprenaline induced a time-dependent and concentration-dependent desensitization of the beta-adrenergic responsiveness without altering that to glucagon (homologous desensitization). The desensitization was evidenced both in the cyclic AMP accumulation and in the stimulation of ureagenesis induced by the beta-adrenergic agonists. Under the same conditions, preincubation with glucagon induced no desensitization. Propranolol was also unable to induce desensitization, but blocked that induced by isoprenaline. Pertussis-toxin treatment did not alter the homologous beta-adrenergic desensitization induced by isoprenaline.
摘要
甲状腺功能减退大鼠的肝细胞具有显著的β-肾上腺素能反应性。用异丙肾上腺素对这些肝细胞进行预孵育可诱导β-肾上腺素能反应性的时间依赖性和浓度依赖性脱敏，而不会改变对胰高血糖素的反应性（同源脱敏）。β-肾上腺素能激动剂诱导的环磷酸腺苷积累和尿素生成刺激均出现了脱敏现象。在相同条件下，用胰高血糖素预孵育未诱导脱敏。普萘洛尔也不能诱导脱敏，但可阻断异丙肾上腺素诱导的脱敏。百日咳毒素处理并未改变异丙肾上腺素诱导的同源β-肾上腺素能脱敏。
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