Andina Diana, Brambilla Davide, Munzinger Noah, Frei Jasmine, Zivko Cristina, Leroux Jean-Christophe, Luciani Paola
Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology (ETHZ), Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
Biologisch-Pharmazeutische Fakultät, Institut für Pharmazie, Friedrich-Schiller-Universität Jena, 07743, Jena, Germany.
Chemistry. 2017 Apr 6;23(20):4765-4769. doi: 10.1002/chem.201700563. Epub 2017 Mar 23.
Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte-specific switch-on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide-nucleic-acid-based linker. The ROS-sensing ability was assessed and validated in cell-free assays and in cell culture.
内源性分析物（如超氧化物）的细胞外检测可为体内平衡和疾病（如肿瘤发生）机制提供重要见解。开发了一种具有荧光参考物和分析物特异性开启染料的比率探针。通过基于模块化肽核酸的连接子连接两个荧光团，确保了细胞外环境中活性氧的检测。在无细胞试验和细胞培养中评估并验证了活性氧传感能力。
