促肾上腺皮质激素（ACTH1 - 24）、环磷酸腺苷和佛波酯（12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯）对合成培养基中兔原代肾上腺皮质细胞DNA复制和增殖的影响。
The effects of corticotrophin (ACTH1-24), cyclic AMP and TPA (12-O-tetradecanoyl phorbol-13-acetate) on DNA replication and proliferation of primary rabbit adrenocortical cells in a synthetic medium.
作者信息
Menapace L, Armato U, Whitfield J F
机构信息
Institute of Human Anatomy and Histology, University of Verona, Venetia, Italy.
出版信息
Biochem Biophys Res Commun. 1987 Nov 13;148(3):1295-303. doi: 10.1016/s0006-291x(87)80274-7.
ACTH1-24 stimulated the parenchymal cells in cultures of rat adrenal cortex in serum-free synthetic HiWoBa 2000 medium to replicate DNA, enter mitosis and divide. But ACTH's principal mediator, cyclic AMP, was not a complete mitogen: the adenylate cyclase-stimulating cholera toxin and dibutyryl cyclic AMP stimulated parenchymal cells to replicate DNA but not to enter mitosis. Thus, there must have been an additional mediator of the response to ACTH1-24 that enabled the parenchymal cells to enter mitosis. This additional mediator might have been protein kinase C because a protein kinase C activator and cyclic AMP elevator, TPA, stimulated the adrenocortical parenchymal cells to replicate DNA, enter mitosis and divide.
促肾上腺皮质激素1-24（ACTH1-24）在无血清合成HiWoBa 2000培养基中刺激大鼠肾上腺皮质培养物中的实质细胞进行DNA复制、进入有丝分裂并分裂。但是促肾上腺皮质激素的主要介质环磷酸腺苷（cAMP）并非完全的促有丝分裂原：刺激腺苷酸环化酶的霍乱毒素和二丁酰环磷酸腺苷刺激实质细胞进行DNA复制，但不会进入有丝分裂。因此，对于ACTH1-24的反应必定存在一种额外的介质，使实质细胞能够进入有丝分裂。这种额外的介质可能是蛋白激酶C，因为蛋白激酶C激活剂和环磷酸腺苷增强剂佛波酯（TPA）刺激肾上腺皮质实质细胞进行DNA复制、进入有丝分裂并分裂。
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