木岛链霉菌 DSM40847 的基因组挖掘作为博来霉素的生产者，为工程设计博来霉素类似物提供生物技术平台。
Genome Mining of Streptomyces mobaraensis DSM40847 as a Bleomycin Producer Providing a Biotechnology Platform To Engineer Designer Bleomycin Analogues.
机构信息
Department of Chemistry, ‡Department of Molecular Medicine, §Natural Products Library Initiative at the Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
出版信息
Org Lett. 2017 Mar 17;19(6):1386-1389. doi: 10.1021/acs.orglett.7b00283. Epub 2017 Mar 3.
Streptomyces mobaraensis DSM40847 has been identified by genome mining and confirmed to be a new bleomycin (BLM) producer. Manipulation of BLM biosynthesis in S. mobaraensis has been demonstrated, as exemplified by the engineered production of 6'-deoxy-BLM A2, providing a biotechnology platform for BLM biosynthesis and engineering. Comparison of DNA cleavage efficiency and kinetics among 6'-deoxy-BLM A2 and selected analogues supports the wisdom of altering the disaccharide moiety to fine-tune BLM activity.
经基因组挖掘鉴定，木岛链霉菌 DSM40847 是一种新的博莱霉素（BLM）产生菌。已证实可对木岛链霉菌中的 BLM 生物合成进行操作，例如工程生产 6'-脱氧 BLM A2，为 BLM 生物合成和工程提供了生物技术平台。6'-脱氧 BLM A2 和选定类似物之间的 DNA 切割效率和动力学比较支持改变二糖部分以微调 BLM 活性的合理性。
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