Morris P L, Vale W W, Bardin C W
Population Council, New York, NY 10021.
Biochem Biophys Res Commun. 1987 Nov 13;148(3):1513-9. doi: 10.1016/s0006-291x(87)80303-0.
Inhibin, a hormone produced by Sertoli cells in response to FSH, regulates androgen production in nearby Leydig cells. Beta-endorphin synthesized by Leydig cells under LH control is also known to regulate Sertoli function. To delineate whether beta-endorphin might constitute part of a short loop regulatory system between these two testicular cells, the effect of this opiate on inhibin secretion was examined. Beta-endorphin alone did not alter basal inhibin accumulation in primary Sertoli cell-enriched cultures, however it did significantly reduce FSH-induced inhibin production and adenylyl cyclase activity but had no effect on forskolin-stimulated inhibin accumulation or adenylyl cyclase activity. Other opioid peptides (ACTH, dMSH, methionine-enkephalin) were without effect. These observations suggest that beta-endorphin regulates inhibin secretion by inhibiting FSH receptor coupling to adenylyl cyclase.
抑制素是支持细胞响应促卵泡激素（FSH）产生的一种激素，可调节附近睾丸间质细胞中的雄激素生成。已知在促黄体生成素（LH）控制下由睾丸间质细胞合成的β-内啡肽也可调节支持细胞的功能。为了阐明β-内啡肽是否可能构成这两种睾丸细胞之间短反馈调节系统的一部分，研究了这种阿片类物质对抑制素分泌的影响。单独的β-内啡肽不会改变原代富含支持细胞的培养物中抑制素的基础积累，然而，它确实显著降低了FSH诱导的抑制素产生和腺苷酸环化酶活性，但对福斯高林刺激的抑制素积累或腺苷酸环化酶活性没有影响。其他阿片肽（促肾上腺皮质激素、d-黑素细胞刺激素、甲硫氨酸脑啡肽）则没有作用。这些观察结果表明，β-内啡肽通过抑制FSH受体与腺苷酸环化酶的偶联来调节抑制素的分泌。
