Hassan Sherif S, Thomann Charity, Ettarh Rajunor, Ahmad Zulfiqar
Department of Medical Education, California University of Sciences and Medicine, School of Medicine (Cal Med-SOM), Colton, California.
Department of Anatomy, Faculty of Medicine, Cairo University, Cairo, Egypt.
Neurourol Urodyn. 2017 Nov;36(8):2003-2010. doi: 10.1002/nau.23249. Epub 2017 Mar 3.
Polymyxin E was used for treating gram-negative bacterial infections but not recently for fear of its nephrotoxicity. Silybin has potential to counteract nephrotoxicity; however, few studies have investigated its protective effect on the kidney in an animal model. The purpose of the present study was to assess whether silybin could decrease elevated urine and serum renal biochemical markers induced by polymyxin E in rat kidney.
Forty rats were divided randomly into four groups of 10 rats: control (I), vehicle (II), treatment (III, using polymyxin E), and protection (IV, using silybin and polymyxin E). Urine was collected daily for 7 days to test for N-acetyl-beta-D-glucosaminidase (NAG). Serum was collected after euthanizing the rats on day 7 to test kidney functions. RESULTS Group III had significant increases in NAG (all P < 0.001) compared with the other groups, but no differences were found between the other groups. Significant differences in kidney functions were found between Group III and Groups I and II, and between Group IV and Groups I and II (all P < 0.001). No differences were found between Groups III and IV.
Group III results suggested an affection of the renal glomeruli and tubules, and Group IV results suggested a possible protective effect of silybin against polymyxin E-induced nephrotoxicity. Additional studies are recommended that use different doses of silybin for Groups III and IV to test for statistical differences for kidney functions and that test the protective effect of silybin against nephrotoxicity induced by polymyxin E in humans.
多粘菌素E曾用于治疗革兰氏阴性菌感染，但由于担心其肾毒性，近来已不再使用。水飞蓟宾有抵消肾毒性的潜力；然而，很少有研究在动物模型中探究其对肾脏的保护作用。本研究的目的是评估水飞蓟宾是否能降低多粘菌素E诱导的大鼠肾脏尿液和血清中肾生化标志物的升高。
40只大鼠随机分为4组，每组10只：对照组（I）、赋形剂组（II）、治疗组（III，使用多粘菌素E）和保护组（IV，使用水飞蓟宾和多粘菌素E）。连续7天每天收集尿液以检测N-乙酰-β-D-氨基葡萄糖苷酶（NAG）。在第7天对大鼠实施安乐死后收集血清以检测肾功能。结果：与其他组相比，III组的NAG显著升高（所有P < 0.001），但其他组之间未发现差异。III组与I组和II组之间以及IV组与I组和II组之间的肾功能存在显著差异（所有P < 0.001）。III组和IV组之间未发现差异。
III组结果提示肾小球和肾小管受到影响，IV组结果提示水飞蓟宾可能对多粘菌素E诱导的肾毒性具有保护作用。建议进一步开展研究，对III组和IV组使用不同剂量的水飞蓟宾，以检测肾功能的统计学差异，并检测水飞蓟宾对人类多粘菌素E诱导的肾毒性的保护作用。
