蛋白激酶C（PkcA）与磷脂酶D（PldB）的功能相互作用调节盘基网柄菌的聚集和发育。
Functional interaction of PkcA and PldB regulate aggregation and development in Dictyostelium discoideum.
作者信息
Singh Sean, Mohamed Wasima, Aguessy Annelie, Dyett Ella, Shah Shriraj, Khan Mohammedasad, Baskar Ramamurthy, Brazill Derrick
机构信息
Department of Biological Sciences, Hunter College and The Graduate Center, The City University of New York, New York, NY, USA.
Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology-Madras, Chennai 600036, India.
出版信息
Cell Signal. 2017 Jun;34:47-54. doi: 10.1016/j.cellsig.2017.02.022. Epub 2017 Feb 28.
Multicellular development in Dictyostelium discoideum involves tightly regulated signaling events controlling the entry into development, initiation of aggregation and chemotaxis, and cellular differentiation. Here we show that PkcA, a Dictyostelium discoideum Protein Kinase C-orthologue, is involved in quorum sensing and the initiation of development, as well as cAMP sensing during chemotaxis. Additionally, by epistasis analysis we provide evidence that PkcA and PldB (a Phospholipase D-orthologue) functionally interact to regulate aggregation, differentiation, and cell-cell adhesion during development. Finally, we show that PkcA acts as a positive regulator of intracellular PLD-activity during development. Taken together, our results suggest that PkcA act through PldB, by regulating PLD-activity, in order to control events during development.
盘基网柄菌的多细胞发育涉及严格调控的信号事件，这些事件控制着发育的起始、聚集和趋化作用的启动以及细胞分化。我们在此表明，盘基网柄菌蛋白激酶C同源物PkcA参与群体感应和发育起始，以及趋化作用期间的cAMP感应。此外，通过上位性分析，我们提供证据表明PkcA和PldB（磷脂酶D同源物）在功能上相互作用，以调节发育过程中的聚集、分化和细胞间粘附。最后，我们表明PkcA在发育过程中作为细胞内PLD活性的正调节因子。综上所述，我们的结果表明，PkcA通过调节PLD活性，经由PldB发挥作用，从而控制发育过程中的各种事件。
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