利用piggyBac转座系统制备转基因绒山羊。
Generation of a transgenic cashmere goat using the piggyBac transposition system.
作者信息
Bai Ding-Ping, Yang Ming-Ming, Qu Lei, Chen Yu-Lin
机构信息
College of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou 350002, China; Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fuzhou 350002, China.
College of Animal Science and Technology, Northwest Agricultural and Forestry University, Yangling 712100, China.
出版信息
Theriogenology. 2017 Apr 15;93:1-6. doi: 10.1016/j.theriogenology.2017.01.036. Epub 2017 Jan 23.
The development of transgenic technologies in the Cashmere goat (Capra hircus) has the potential to improve the quality of the meat and wool. The piggyBac (PB) transposon system is highly efficient and can be used to transpose specific target genes into the genome. Here, we developed a PB transposon system to produce transgenic Cashmere goat fetal fibroblasts (GFFs) with the enhanced green fluorescent protein (EGFP). We then used the genetically modified GFFs as nuclear donors to generate transgenic embryos by somatic cell nuclear transfer (SCNT). The embryos (n = 40) were implanted into female goats (n = 20). One transgenic kid that expressed EGFP throughout the surface features of its body was born. This result demonstrated the usefulness of PB transposon system in generating transgenic Cashmere goats.
在绒山羊（Capra hircus）中开发转基因技术有潜力提高肉和羊毛的质量。piggyBac（PB）转座子系统效率很高，可用于将特定目标基因转座到基因组中。在此，我们开发了一种PB转座子系统，以产生带有增强型绿色荧光蛋白（EGFP）的转基因绒山羊胎儿成纤维细胞（GFFs）。然后，我们使用经过基因改造的GFFs作为核供体，通过体细胞核移植（SCNT）产生转基因胚胎。将这些胚胎（n = 40）植入母羊（n = 20）体内。诞生了一只在身体表面特征上都表达EGFP的转基因小羊。这一结果证明了PB转座子系统在培育转基因绒山羊方面的实用性。
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