Perera K U E, Wickramasinghe Susiji, Perera B V P, Bandara K B A T, Rajapakse R P V J
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka.
Department of Parasitology, Faculty of Medicine, University of Peradeniya, Sri Lanka.
Parasitol Int. 2017 Jun;66(3):279-286. doi: 10.1016/j.parint.2017.02.007. Epub 2017 Feb 28.
The present work provides a detailed morphological and molecular description of Anoplocephala manubriata in elephants. Adult worms were recovered during an autopsy of a wild elephant in Elephant Transit Home, Udawalawe, Sri Lanka. Necropsy findings revealed a severe cestode infection in the small intestine. These tapeworms were tightly attached to the intestinal mucosae, resulted in hyperemic thickened intestinal mucosae, variable size irregular well-demarcated multifocal ulcerative regions sometimes covered with necrotic membranes and variable size, diffuse, well-demarcated raised nodular masses were evident in the small intestine. The article provides an account of the biology of A. manubriata and a comparative analysis of the morphology and morphometrics of Anoplocephala species that occur in different hosts. Phylogenetic analysis of the second internal transcribed spacer region (ITS-2), a portion of the 28S region and cytochrome oxidase subunit 1 (COX1) genes revealed that A. manubriata is closely associated with Anoplocephala species in horse in comparison to other Anoplocephalines. This study will enhance the current knowledge in taxonomy of elephant tapeworms and contribute to future phylogenetic studies.
本研究对大象体内的马氏裸头绦虫进行了详细的形态学和分子描述。成虫是在斯里兰卡乌达瓦拉韦大象中转之家对一头野生大象进行尸检时发现的。尸检结果显示小肠存在严重的绦虫感染。这些绦虫紧密附着于肠黏膜，导致肠黏膜充血增厚，有时可见大小不一、边界清晰的多灶性溃疡性区域，表面覆盖坏死膜，小肠内还可见大小不一、弥漫性、边界清晰的隆起结节状肿块。本文介绍了马氏裸头绦虫的生物学特性，并对不同宿主中出现的裸头绦虫种类的形态和形态测量进行了比较分析。对第二内部转录间隔区（ITS-2）、28S区域的一部分以及细胞色素氧化酶亚基1（COX1）基因的系统发育分析表明，与其他裸头绦虫相比，马氏裸头绦虫与马体内的裸头绦虫种类关系密切。这项研究将增进目前对大象绦虫分类学的认识，并为未来的系统发育研究做出贡献。
