含左旋咪唑递送系统的微乳剂对与蓝氏贾第鞭毛虫相互作用的血液吞噬细胞的体外免疫调节作用

In vitro immunomodulatory effects of microemulsions with levamisole delivery systems on blood phagocytes interacting with Giardia lamblia.

作者信息

Hernandes Mara Rosa Gil, Moraes Lucélia Campelo Albuquerque, Ribeiro Elton Brito, Fagundes Danny Laura Gomes, Honorio-França Adenilda Cristina, França Eduardo Luzía

机构信息

Department of Parasitology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Institute of Biological and Health Science - Federal University of Mato Grosso, Barra do Garças, MT, Brazil.

Institute of Biological and Health Science - Federal University of Mato Grosso, Barra do Garças, MT, Brazil.

出版信息

Parasitol Int. 2017 Jun;66(3):299-304. doi: 10.1016/j.parint.2017.02.005. Epub 2017 Feb 28.

DOI:10.1016/j.parint.2017.02.005

PMID:28257952

Abstract

BACKGROUND

Giardiasis is one of the main parasites that infect the gastrointestinal tract of humans, affecting hundreds of millions of people worldwide, particularly in developing countries. Antiparasitics administered to treat giardiasis are inefficient in 20% of the cases, usually because of parasite resistance and side effects. In this scenario, microemulsions are a promising pharmaceutical alternative as carriers of molecules with therapeutic action that stimulate the immune system.

METHODS

The study evaluated the effects of a microemulsion delivery system with levamisole hydrochloride on the functional activity of MN phagocytes incubated with G. lamblia.

RESULTS

The microemulsion formulated was incorporated with levamisole hydrochloride using distilled water, caprylic/capric triglyceride-Polymol 812®, Sorbitan Oleate-Span 80®, Polysorbate 80 - Tween 80® and 1-butanol. The activity of the microemulsion was analyzed by phagocytosis rate, microbicidal activity, apoptosis rate and intracellular calcium concentration. Phagocytosis rate, microbicidal activity and apoptosis index increased in the microemulsion treatment. The results suggest that the microemulsion improves the therapeutic efficacy of levamisole, increasing the functional activity of phagocytes.

CONCLUSIONS

The microemulsion with a levamisole delivery system is therefore an efficient alternative for treating giardiasis, acting as an immunomodulator that probably causes fewer side effects than conventional drugs.

摘要

背景

贾第虫病是感染人类胃肠道的主要寄生虫病之一，全球数亿人受其影响，尤其是在发展中国家。用于治疗贾第虫病的抗寄生虫药物在20%的病例中疗效不佳，通常是由于寄生虫耐药性和副作用。在这种情况下，微乳剂作为具有治疗作用且能刺激免疫系统的分子载体，是一种有前景的药物替代品。

方法

本研究评估了含盐酸左旋咪唑的微乳递送系统对与蓝氏贾第鞭毛虫共孵育的MN吞噬细胞功能活性的影响。

结果

所制备的微乳剂由蒸馏水、辛酸/癸酸甘油三酯 - Polymol 812®、油酸山梨坦 - Span 80®、聚山梨酯80 - Tween 80®和正丁醇与盐酸左旋咪唑混合而成。通过吞噬率、杀菌活性、凋亡率和细胞内钙浓度分析微乳剂的活性。微乳剂处理组的吞噬率、杀菌活性和凋亡指数均升高。结果表明，微乳剂提高了左旋咪唑的治疗效果，增强了吞噬细胞的功能活性。