Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, Burjassot, Valencia 46100, Spain.
Departamento de Bioquímica y Biología Molecular, Universidad de Valencia, Burjassot, Valencia 46100, Spain.
Biochim Biophys Acta Gene Regul Mech. 2017 Jul;1860(7):803-811. doi: 10.1016/j.bbagrm.2017.02.009. Epub 2017 Feb 28.
Iwr1 is an RNA polymerase II (RNPII) interacting protein that directs nuclear import of the enzyme which has been previously assembled in the cytoplasm. Here we present genetic and molecular evidence that links Iwr1 with transcription. Our results indicate that Iwr1 interacts with RNPII during elongation and is involved in the disassembly of the enzyme from chromatin. This function is especially important in resolving problems posed by damage-arrested RNPII, as shown by the sensitivity of iwr1 mutants to genotoxic drugs and the Iwr1's genetic interactions with RNPII degradation pathway mutants. Moreover, absence of Iwr1 causes genome instability that is enhanced by defects in the DNA repair machinery.
Iwr1 是一种 RNA 聚合酶 II（RNPII）相互作用蛋白，可指导已在细胞质中组装的酶的核输入。在这里，我们提供了遗传和分子证据，将 Iwr1 与转录联系起来。我们的结果表明，Iwr1 在延伸过程中与 RNPII 相互作用，并参与酶从染色质上的解组装。正如 iwr1 突变体对遗传毒性药物的敏感性以及 Iwr1 与 RNPII 降解途径突变体的遗传相互作用所表明的那样，这种功能在解决由 RNA 聚合酶 II 阻滞引起的问题方面尤为重要。此外，缺乏 Iwr1 会导致基因组不稳定，而 DNA 修复机制的缺陷会增强这种不稳定性。
