Lalmahomed Z S, Coebergh van den Braak R R J, Oomen M H A, Arshad S P, Riegman P H J, IJzermans J N M
Department of Surgery, Erasmus MC Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
Department of Pathology, Erasmus MC Medical Center, Rotterdam, The Netherlands.
Cell Tissue Bank. 2017 Sep;18(3):425-431. doi: 10.1007/s10561-017-9613-x. Epub 2017 Mar 3.
The growing interest in the molecular subclassification of colorectal cancers is increasingly facilitated by large multicenter biobanking initiatives. The quality of tissue sampling is pivotal for successful translational research. This study shows the quality of fresh frozen tissue sampling within a multicenter cohort study for colorectal cancer (CRC) patients. Each of the seven participating hospitals randomly contributed ten tissue samples, which were collected following Standard Operating Procedures (SOP) using established techniques. To indicate if the amount of intact RNA is sufficient for molecular discovery research and prove SOP compliance, the RNA integrity number (RIN) was determined. Samples with a RIN < 6 were measured a second time and when consistently low a third time. The highest RIN was used for further analysis. 91% of the tissue samples had a RIN ≥ 6 (91%). The remaining six samples had a RIN between 5 and 6 (4.5%) or lower than 5 (4.5%). The median overall RIN was 7.3 (range 2.9-9.0). The median RIN of samples in the university hospital homing the biobank was 7.7 and the median RIN for the teaching hospitals was 7.3, ranging from 6.5 to 7.8. No differences were found in the outcome of different hospitals (p = 0.39). This study shows that the collection of high quality fresh frozen samples of colorectal cancers is feasible in a multicenter design with complete SOP adherence. Thus, using basic sampling techniques large patient cohorts can be organized for predictive and prognostic (bio)marker research for CRC.
大型多中心生物样本库计划越来越有助于人们对结直肠癌分子亚分类的兴趣日益增长。组织采样的质量对于成功的转化研究至关重要。本研究展示了在一项针对结直肠癌（CRC）患者的多中心队列研究中新鲜冷冻组织采样的质量。七家参与研究的医院每家随机提供了10个组织样本，这些样本是按照标准操作程序（SOP）采用既定技术收集的。为了表明完整RNA的量是否足以进行分子发现研究并证明符合SOP，测定了RNA完整性数值（RIN）。RIN < 6的样本进行了第二次测量，如果结果持续较低则进行第三次测量。使用最高的RIN进行进一步分析。91%的组织样本RIN≥6（91%）。其余六个样本的RIN在5至6之间（4.5%）或低于5（4.5%）。总体RIN的中位数为7.3（范围2.9 - 9.0）。设有生物样本库的大学医院样本的RIN中位数为7.7，教学医院样本的RIN中位数为7.3，范围在6.5至7.8之间。不同医院的结果未发现差异（p = 0.39）。本研究表明，在完全遵守SOP的多中心设计中，收集高质量的结直肠癌新鲜冷冻样本是可行的。因此，使用基本采样技术可以组织大型患者队列用于CRC的预测和预后（生物）标志物研究。
