Xu Huihui, Zhang Yang, Feng Xin, Tie Kunyuan, Cao Yuan, Han Wenyu
College of Veterinary Medicine, Jilin University, Xi'an Road 5333#, Changchun, 130062, People's Republic of China.
Biotechnol Lett. 2017 Jun;39(6):897-903. doi: 10.1007/s10529-017-2312-7. Epub 2017 Mar 3.
To identify and characterize a novel antimicrobial peptide, catesbeianin-1.
Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 exhibited moderate hemolytic activity (approx 8%) at 100 μg/ml, and its HC (50% hemolytic concentration) was 300 μg/ml. Its cytotoxicity was approx 10-20% at 100 μg/ml, and its CC (50% cytotoxic concentration) was >100 μg/ml. The LD of catesbeianin-1 in mice was 80 mg/kg. At 3.1 µg/ml, catesbeianin-1 significantly inhibited the growth of methicillin-resistant Staphylococcus aureus.
A new antimicrobial peptide from the skin of Lithobates catesbeianus (American bullfrog) may represent a template for the development of novel antimicrobial agents.
鉴定并表征一种新型抗菌肽——牛蛙抗菌肽-1。
牛蛙抗菌肽-1由25个氨基酸组成，呈α螺旋结构，具有阳离子性和两亲性。它对革兰氏阳性菌和革兰氏阴性菌均具有抗菌活性。它对胰蛋白酶和胃蛋白酶具有抗性。牛蛙抗菌肽-1在100μg/ml时表现出中等程度的溶血活性（约8%），其HC（50%溶血浓度）为300μg/ml。在100μg/ml时其细胞毒性约为10 - 20%，其CC（50%细胞毒性浓度）>100μg/ml。牛蛙抗菌肽-1在小鼠体内的半数致死量为80mg/kg。在3.1μg/ml时，牛蛙抗菌肽-1显著抑制耐甲氧西林金黄色葡萄球菌的生长。
来自牛蛙皮肤的一种新型抗菌肽可能为新型抗菌剂的开发提供模板。
