Alves Raul Santos, Alves Levy Bueno, Altoé Luciana Schulthais, Sarandy Mariáurea Matias, Freitas Mariella Bontempo, Silveira Nelson José Freitas, Novaes Rômulo Dias, Gonçalves Reggiani Vilela
Department of General Biology, Federal University of Viçosa, Viçosa, 36570-900 Minas Gerais, Brazil.
Laboratory of Molecular Modeling and Computer Simulation-MolMod-CS, Institute of Chemistry, Federal University of Alfenas, Alfenas, 37130-001 Minas Gerais, Brazil.
Oxid Med Cell Longev. 2020 Oct 23;2020:4352761. doi: 10.1155/2020/4352761. eCollection 2020.
Skin wounds are closely correlated with opportunistic infections and sepsis risk. Due to the need of more efficient healing drugs, animal peptides are emerging as new molecular platforms to accelerate skin wound closure and to prevent and control bacterial infection.
The aim of this study was to evaluate the preclinical evidence on the impact of animal peptides on skin wound healing. In addition, we carried out a critical analysis of the studies' methodological quality. . This systematic review was performed according to the PRISMA guidelines, using a structured search on the PubMed-Medline, Scopus, and Web of Science platforms to retrieve studies published until August 25, 2020 at 3 : 00 pm. The studies included were limited to those that used animal models, investigated the effect of animal peptides with no association with other compounds on wound healing, and that were published in English. Bias analysis and methodological quality assessments were examined through the SYRCLE's RoB tool.
Thirty studies were identified using the PRISMA workflow. In general, animal peptides were effective in accelerating skin wound healing, especially by increasing cellular proliferation, neoangiogenesis, colagenogenesis, and reepithelialization. Considering standardized methodological quality indicators, we identified a marked heterogeneity in research protocols and a high risk of bias associated with limited characterization of the experimental designs.
Animal peptides show a remarkable healing potential with biotechnological relevance for regenerative medicine. However, rigorous experimental approaches are still required to clearly delimit the mechanisms underlying the healing effects and the risk-benefit ratio attributed to peptide-based treatments.
皮肤伤口与机会性感染及败血症风险密切相关。由于需要更有效的愈合药物，动物肽正成为加速皮肤伤口愈合以及预防和控制细菌感染的新分子平台。
本研究旨在评估关于动物肽对皮肤伤口愈合影响的临床前证据。此外，我们对这些研究的方法学质量进行了批判性分析。本系统评价按照PRISMA指南进行，在PubMed-Medline、Scopus和Web of Science平台上进行结构化检索，以获取截至2020年8月25日下午3点发表的研究。纳入的研究仅限于使用动物模型、研究与其他化合物无关联的动物肽对伤口愈合影响且以英文发表的研究。通过SYRCLE的RoB工具进行偏倚分析和方法学质量评估。
使用PRISMA工作流程识别出30项研究。总体而言，动物肽在加速皮肤伤口愈合方面有效，尤其是通过增加细胞增殖、新生血管形成、胶原生成和上皮再形成。考虑到标准化的方法学质量指标，我们发现研究方案存在显著异质性，且与实验设计特征有限相关的偏倚风险较高。
动物肽在再生医学方面具有显著的愈合潜力和生物技术相关性。然而，仍需要严格的实验方法来明确界定愈合效果的潜在机制以及基于肽的治疗的风险效益比。
