低氧诱导因子-2α 通过 MAPK 信号通路影响 MG-63 骨肉瘤细胞的增殖和凋亡。
HIF-2α affects proliferation and apoptosis of MG-63 osteosarcoma cells through MAPK signaling.
机构信息
Department of Orthopedic Surgery, The Affiliated Hospital of Logistics College of Chinese People's Armed Police Force, Pingjin Hospital, Tianjin 300162, P.R. China.
Department of Neurological Intensive Care Unit, Tianjin Huanhu Hospital, Tianjin 300060, P.R. China.
出版信息
Mol Med Rep. 2017 Apr;15(4):2174-2178. doi: 10.3892/mmr.2017.6243. Epub 2017 Feb 23.
The present study explored the mechanism of hypoxia-inducible factor (HIF)‑2α in proliferation and apoptosis of the osteosarcoma cell line, MG‑63. Cells were treated with small interfering RNA (siRNA) against HIF‑2α (silenced group) or without siRNA (control group). Cell viability of MG‑63 in the silenced and the control groups was determined by MTT assay; cell apoptosis was measured by flow cytometry; the expression of HIF‑2α and mitogen-activated protein kinase (MAPK)-p38 were measured by western blotting. According to MTT assay, 48 h after siRNA transfection, compared with the control group, cells in the silenced group significantly declined in quantity and the number of apoptotic cells increased significantly. The expression of HIF‑2α and MAPK‑p38 were significantly decreased (P<0.05). In conclusion, knockdown of HIF-2α in the osteosarcoma cell line reduced the proliferation of cancer cells and increased apoptosis. These effects likely occurred through the MAPK‑p38 signaling pathway.
本研究探讨了低氧诱导因子（HIF）-2α 在骨肉瘤细胞系 MG-63 增殖和凋亡中的作用机制。用 HIF-2α 的小干扰 RNA（siRNA）（沉默组）或无 siRNA（对照组）处理细胞。通过 MTT 法测定沉默组和对照组中 MG-63 细胞的存活率；通过流式细胞术测量细胞凋亡；通过 Western blot 测定 HIF-2α 和丝裂原活化蛋白激酶（MAPK）-p38 的表达。根据 MTT 法，在 siRNA 转染后 48 h，与对照组相比，沉默组细胞数量明显减少，凋亡细胞数量明显增加。HIF-2α 和 MAPK-p38 的表达明显降低（P<0.05）。综上所述，骨肉瘤细胞系中 HIF-2α 的敲低降低了癌细胞的增殖并增加了细胞凋亡。这些作用可能通过 MAPK-p38 信号通路发生。
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