Department of Spine Surgery, PLA No. 252 Hospital, Baoding, Hebei 071000, P.R. China.
Department of Rehabilitation, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
Mol Med Rep. 2017 Apr;15(4):2241-2246. doi: 10.3892/mmr.2017.6261. Epub 2017 Feb 28.
The aim of the present study was to determine whether amyloid-β (Aβ) protein could be detected in the serum and cerebrospinal fluid (CSF) of rats with traumatic spinal cord injury (SCI) and whether Aβ protein levels in serum and CSF correlated with the injury severity. A total of 140 adult female Sprague‑Dawley rats were randomly divided into four groups: Sham, mild injury, moderate injury and severe injury. Serum and CSF samples were collected at 12 h, 1, 3, 7, 14, 21 and 28 days post‑injury. ELISA analysis for serum and CSF Aβ protein was performed. Locomotor function of all animals was assessed using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. Following SCI, the protein levels of Aβ in serum and CSF samples from SCI groups significantly increased from 12 h (P<0.05) and peaked 3 days after injury. A significant increase of Aβ levels in serum and CSF in the severe SCI group was also observed at 28 days after injury (P<0.05). At 28 days after injury the protein levels of Aβ in serum and CSF were significantly correlated with the severity of injury (serum, R2=‑0.806, P<0.01; CSF, R2=‑0.694, P<0.01). A significant correlation between Aβ protein level in serum and CSF and neurological deficits (BBB score) was also observed (P<0.01). The protein level of Aβ in serum and CSF was severity and time‑dependent during the acute phase in rats with traumatic SCI. Monitoring the level of Aβ protein in serum may improve the evaluation of SCI severity and the neuron functional status following SCI.
本研究旨在确定创伤性脊髓损伤（SCI）大鼠的血清和脑脊液（CSF）中是否可检测到淀粉样β（Aβ）蛋白，以及血清和 CSF 中的 Aβ 蛋白水平是否与损伤严重程度相关。总共 140 只成年雌性 Sprague-Dawley 大鼠随机分为四组：假手术组、轻度损伤组、中度损伤组和重度损伤组。在损伤后 12 h、1、3、7、14、21 和 28 天收集血清和 CSF 样本。采用 ELISA 分析检测血清和 CSF 中 Aβ 蛋白的含量。采用 Basso、Beattie 和 Bresnahan（BBB）运动评分量表对所有动物的运动功能进行评估。在 SCI 后，SCI 组的血清和 CSF 样本中的 Aβ 蛋白水平从 12 h 开始显著增加（P<0.05），并在损伤后 3 天达到峰值。在损伤后 28 天，重度 SCI 组的血清和 CSF 中 Aβ 水平也显著升高（P<0.05）。在损伤后 28 天，血清和 CSF 中 Aβ 蛋白水平与损伤严重程度显著相关（血清，R2=‑0.806，P<0.01；CSF，R2=‑0.694，P<0.01）。同时，还观察到血清和 CSF 中 Aβ 蛋白水平与神经功能缺损（BBB 评分）之间存在显著相关性（P<0.01）。在创伤性 SCI 大鼠的急性期，血清和 CSF 中 Aβ 蛋白水平与损伤严重程度和时间呈依赖性。监测血清中 Aβ 蛋白的水平可能有助于评估 SCI 的严重程度以及 SCI 后的神经元功能状态。
